tpj12363-sup-0001-TableS1-MethodsS1-S9.docWord document117K

Table S1. List of primers used in real-time quantitative RT-PCR in this study.

Methods S1. Plant materials and plant transformation.

Methods S2. Construction of chimeric GUS constructs.

Methods S3. Construction of the 35S:AGL13 RNA interference construct.

Methods S4. Construction of the AGL13 + SRDX and SEP2 + SRDX constructs.

Methods S5. RT-PCR and real-time PCR analysis.

Methods S6. Microscopy and semi-thin sections.

Methods S7. GUS staining, pollen staining and pollen adhesion assay.

Methods S8. Construction of FRET-associated fusion constructs.

Methods S9. The confocal laser scanning microscope imaging and FRET assay.


Figure S1. AGL13-GUS reporter gene fusions.

Figure S2. GUS staining patterns in transgenic Arabidopsis plants.

Figure S3. GUS staining patterns in the anther of 13P-1-GUS flower.

Figure S4. GUS staining patterns in carpel of 13P-1-GUS flower.

Figure S5. Confocal laser scanning microscopy (CLSM) and scanning electron micrographs (SEM) of pollen produced in wild-type and 35S:AGL13 RNAi flowers.

Figure S6. Pollen adhesion assay, semi-thin sections of pollen and analysis of ovule formation for wild-type and 35S:AGL13 RNAi plants.

Figure S7. Confocal laser scanning microscopy (CLSM) of ovules in wild-type and 35S:AGL13 RNAi flowers.

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