AGAMOUS-LIKE13, a putative ancestor for the E functional genes, specifies male and female gametophyte morphogenesis
Article first published online: 6 DEC 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 77, Issue 1, pages 1–15, January 2014
How to Cite
Hsu, W.-H., Yeh, T.-J., Huang, K.-Y., Li, J.-Y., Chen, H.-Y. and Yang, C.-H. (2014), AGAMOUS-LIKE13, a putative ancestor for the E functional genes, specifies male and female gametophyte morphogenesis. The Plant Journal, 77: 1–15. doi: 10.1111/tpj.12363
- Issue published online: 20 DEC 2013
- Article first published online: 6 DEC 2013
- Accepted manuscript online: 24 OCT 2013 11:31AM EST
- Manuscript Accepted: 18 OCT 2013
- Manuscript Revised: 9 OCT 2013
- Manuscript Received: 21 AUG 2013
- National Science Council. Grant Numbers: NSC96–2752-B-005–007-PAE, NSC 98–2321-B-005–007-MY3
- Ministry of Education
Table S1. List of primers used in real-time quantitative RT-PCR in this study.
Methods S1. Plant materials and plant transformation.
Methods S2. Construction of chimeric GUS constructs.
Methods S3. Construction of the 35S:AGL13 RNA interference construct.
Methods S4. Construction of the AGL13 + SRDX and SEP2 + SRDX constructs.
Methods S5. RT-PCR and real-time PCR analysis.
Methods S6. Microscopy and semi-thin sections.
Methods S7. GUS staining, pollen staining and pollen adhesion assay.
Methods S8. Construction of FRET-associated fusion constructs.
Methods S9. The confocal laser scanning microscope imaging and FRET assay.
Figure S1. AGL13-GUS reporter gene fusions.
Figure S2. GUS staining patterns in transgenic Arabidopsis plants.
Figure S3. GUS staining patterns in the anther of 13P-1-GUS flower.
Figure S4. GUS staining patterns in carpel of 13P-1-GUS flower.
Figure S5. Confocal laser scanning microscopy (CLSM) and scanning electron micrographs (SEM) of pollen produced in wild-type and 35S:AGL13 RNAi flowers.
Figure S6. Pollen adhesion assay, semi-thin sections of pollen and analysis of ovule formation for wild-type and 35S:AGL13 RNAi plants.
Figure S7. Confocal laser scanning microscopy (CLSM) of ovules in wild-type and 35S:AGL13 RNAi flowers.
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