SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
tpj12363-sup-0001-TableS1-MethodsS1-S9.docWord document117K

Table S1. List of primers used in real-time quantitative RT-PCR in this study.

Methods S1. Plant materials and plant transformation.

Methods S2. Construction of chimeric GUS constructs.

Methods S3. Construction of the 35S:AGL13 RNA interference construct.

Methods S4. Construction of the AGL13 + SRDX and SEP2 + SRDX constructs.

Methods S5. RT-PCR and real-time PCR analysis.

Methods S6. Microscopy and semi-thin sections.

Methods S7. GUS staining, pollen staining and pollen adhesion assay.

Methods S8. Construction of FRET-associated fusion constructs.

Methods S9. The confocal laser scanning microscope imaging and FRET assay.

tpj12363-sup-0002-FigureS1-S7.pdfapplication/PDF6341K

Figure S1. AGL13-GUS reporter gene fusions.

Figure S2. GUS staining patterns in transgenic Arabidopsis plants.

Figure S3. GUS staining patterns in the anther of 13P-1-GUS flower.

Figure S4. GUS staining patterns in carpel of 13P-1-GUS flower.

Figure S5. Confocal laser scanning microscopy (CLSM) and scanning electron micrographs (SEM) of pollen produced in wild-type and 35S:AGL13 RNAi flowers.

Figure S6. Pollen adhesion assay, semi-thin sections of pollen and analysis of ovule formation for wild-type and 35S:AGL13 RNAi plants.

Figure S7. Confocal laser scanning microscopy (CLSM) of ovules in wild-type and 35S:AGL13 RNAi flowers.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.