Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry
Article first published online: 29 NOV 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 77, Issue 1, pages 160–172, January 2014
How to Cite
Li, M., Baughman, E., Roth, M. R., Han, X., Welti, R. and Wang, X. (2014), Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry. The Plant Journal, 77: 160–172. doi: 10.1111/tpj.12365
- Issue published online: 20 DEC 2013
- Article first published online: 29 NOV 2013
- Accepted manuscript online: 24 OCT 2013 11:31AM EST
- Manuscript Accepted: 15 OCT 2013
- Manuscript Received: 14 SEP 2013
- Manuscript Revised: 14 SEP 2013
- United States Department of Energy (DOE)
- Office of Science
- Office of Basic Energy Sciences (BES)
- Materials Sciences and Engineering Division. Grant Number: # DE-SC0001295
- National Science Foundation. Grant Numbers: MCB-0922879, MCB-0920663, DBI-0521587
Figure S1. Fatty acyl composition of Arabidopsis seeds.
Figure S2. Fragmentation patterns of ammoniated ions of intact TAG molecules.
Figure S3. Measured adjustment factors for commercially available TAGs and deduction of adjustment factors for TAGs with 0 and 3 double bonds.
Figure S4. Derivation of adjustment factors for TAGs with six fatty acyl double bonds (TAG:6).
Figure S5. Derivation of the regression curves for deduction of the adjustment factors at TAG groups of C48, C50, C52, C54, C56, C58, and C60.
Figure S6. Measured adjustment factors for commercially available TAGs and deduced adjustment factors for seed oil TAGs.
Figure S7. The abundance of 10 fatty acyl chains in TAGs at m/z 860.9 and m/z 984.9.
Figure S8. Characterization of Arabidopsis mutant seeds overexpressing pPLAIIIδ.
Figure S9. Levels of individual TAG species in Arabidopsis wild-type and pPLAIIIδ overexpressing seeds.
Table S1. Measured and deduced adjustment factors for commercially available TAGs and seed oil TAGs at 46 m/z.
Table S2. The abundance of 10 fatty acyl chains in each TAG m/z group in Arabidopsis seed oil extract.
Table S3. Levels of TAG molecular species in wild-type and pPLAIIIδ overexpressing seeds of Arabidopsis.
Table S4. Fatty acyl combination analysis of seed oil TAGs.
Method S1. Derivation of the regression curves for deduction of the adjustment factors at 46 seed oil TAG m/zs.
Method S2. Derivation of the formulae for calculation of the fatty acyl combinations at each TAG m/z.
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