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tpj12374-sup-0001-FigS1-S16.pdfapplication/PDF4656K

Figure S1. Multiple alignment of expansin protein sequences by clustalw.

Figure S2. Root hair-specific expression analysis of RH101 and RH102 in response to infection by Mesorhizobium loti.

Figure S3. Promoter sequences of LjEXP7 and LjEXPA8.

Figure S4. Histochemical localization of GUS activity in wild-type roots transformed with the pEpi::GUS+ vector.

Figure S5. Rhizobial infection and nodule organogenesis phenotypes of wild-type roots transformed with the pEpi::GUS+ vector.

Figure S6. Expression analysis of symbiosis genes in root hairs, stripped roots and whole roots of Lotus japonicus.

Figure S7. Rhizobial infection and nodule organogenesis phenotypes of nup85–2, nup133–3, castor–4, pollux–3 and ccamk–3 mutant roots transformed with the empty vector.

Figure S8. Rhizobial infection and nodule organogenesis phenotypes of nfr1–4, nfr5–2, nsp1–1 and nsp2–1 mutant roots transformed with the empty vector.

Figure S9. Stacked histograms comparing frequency counts in terms of the number of nodules or bumps per mutant plants transformed with CCaMK, CASTOR, POLLUX, NUP85 and NUP133 driven by pEpi or p35S.

Figure S10. Spontaneous nodulation phenotypes of wild-type roots transformed with CCaMKT265D driven by pEpi or p35S.

Figure S11. Complementation tests of rhizobial infection and nodule organogenesis phenotypes of nup85–2, nup133–3, castor–4 and pollux–3 mutants transformed with the corresponding genes driven by p35S.

Figure S12. Stacked histograms comparing frequency counts in terms of the number of nodules or bumps per mutant plant by co-transformation with CCaMK under the control of pEpi and truncated CCaMK1–314 or CCaMK1–340 under the control of p35S.

Figure S13. Spontaneous nodule-like structure of the ccamk–3 mutant by co-transformation with CCaMK under the control of pEpi and truncated CCaMK1–314 under the control of p35S.

Figure S14. Complementation tests of rhizobial infection and nodule organogenesis phenotypes of the cyclops–3 mutant transformed with CYCLOPS driven by pEpi or p35S.

Figure S15. Stacked histograms comparing frequency counts in terms of the number of nodules or bumps per mutant plant transformed with CYCLOPS, NSP1, NSP2, NFR1 or NFR5 driven by pEpi or p35S.

Figure S16. Complementation tests of rhizobial infection and nodule organogenesis phenotypes of nsp1–1 and nsp2–1 mutants transformed with the corresponding genes driven by pEpi or p35S.

tpj12374-sup-0002-MethodS1-S2.pdfapplication/PDF50K

Methods S1. Plasmid construction.

Methods S2. RNA isolation from root hairs.

tpj12374-sup-0003-TableS1-S3.pdfapplication/PDF30K

Table S1. Spontaneous nodulation phenotypes.

Table S2. Primer sequences used for construction.

Table S3. Primer sequences used in the expression analysis.

tpj12374-sup-0004-Legends.docxWord document53K*

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