The AAT1 locus is critical for the biosynthesis of esters contributing to ‘ripe apple’ flavour in ‘Royal Gala’ and ‘Granny Smith’ apples
Article first published online: 23 MAY 2014
© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd
The Plant Journal
Volume 78, Issue 6, pages 903–915, June 2014
How to Cite
Souleyre, E. J. F., Chagné, D., Chen, X., Tomes, S., Turner, R. M., Wang, M. Y., Maddumage, R., Hunt, M. B., Winz, R. A., Wiedow, C., Hamiaux, C., Gardiner, S. E., Rowan, D. D. and Atkinson, R. G. (2014), The AAT1 locus is critical for the biosynthesis of esters contributing to ‘ripe apple’ flavour in ‘Royal Gala’ and ‘Granny Smith’ apples. The Plant Journal, 78: 903–915. doi: 10.1111/tpj.12518
- Issue published online: 11 JUN 2014
- Article first published online: 23 MAY 2014
- Accepted manuscript online: 24 MAR 2014 02:07PM EST
- Manuscript Accepted: 17 MAR 2014
- Manuscript Revised: 16 JAN 2014
- Manuscript Received: 7 OCT 2013
- New Zealand Ministry of Business, Innovation and Employment
- PFR internal investment
Figure S1. Western analysis of AAT1 after transient expression in tobacco leaves and in ‘Granny Smith’ and ‘Royal Gala’ ripe fruit cortex and skin.
Figure S2. Homology modelling of AAT1-RGa.
Figure S3.AAT1-RGa gene-specific expression profiles in transgenic ‘Royal Gala’ apple and control lines.
Figure S4. Expression profiles of genes from fatty acid and isoleucine biosynthesis pathways in transgenic ‘Royal Gala’ apple and control lines.
Table S1. Detection of quantitative trait loci for ester and alcohol compounds in fruit from the ‘Royal Gala’ × ‘Granny Smith’ segregating population.
Table S2. Fine mapping of the 2-methylbutyl acetate quantitative trait locus at the top of linkage group 2.
Table S3. Candidate genes within the 635.5-kb interval surrounding the major ester quantitative trait locus on linkage group 2 within the ‘Golden Delicious’ genome.
Table S4. ‘Golden Delicious’ gene models with significant sequence homology to MpAAT1.
Table S5. Genome organisation of the gene models with significant sequence homology to MpAAT1 in ‘Golden Delicious’.
Table S6. Primer sequences used for isolation of AAT1 variants from ‘Granny Smith’ and ‘Royal Gala’.
Table S7. Primer sequences used for quantitative RT-PCR analysis and their predicted product sizes.
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