Both authors contributed equally to this work.
A unique Ni2+ -dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response
Version of Record online: 23 MAY 2014
© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd
The Plant Journal
Volume 78, Issue 6, pages 951–963, June 2014
How to Cite
Mustafiz, A., Ghosh, A., Tripathi, A. K., Kaur, C., Ganguly, A. K., Bhavesh, N. S., Tripathi, J. K., Pareek, A., Sopory, S. K. and Singla-Pareek, S. L. (2014), A unique Ni2+ -dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response. The Plant Journal, 78: 951–963. doi: 10.1111/tpj.12521
- Issue online: 11 JUN 2014
- Version of Record online: 23 MAY 2014
- Accepted manuscript online: 24 MAR 2014 07:34PM EST
- Manuscript Accepted: 19 MAR 2014
- Manuscript Revised: 7 MAR 2014
- Manuscript Received: 31 JAN 2014
- International Centre for Genetic Engineering and Biotechnology (ICGEB)
- Department of Biotechnology (DBT), Government of India
Figure S1. Multiple sequence alignment of the two GLY I domains of OsGLYI-11.2 showing the conserved features, with corresponding GLY I domains from diverse species.
Figure S2. Determination of oligomeric state of OsGLYI-11.2 by gel permeation chromatography.
Figure S3. Heterologous expression of OsGLYI-11.2 provides multiple stress tolerance to Escherichia coli.
Appendix S1. Purification and determination of the oligomeric status of the WT and mutant recombinant proteins by gel permeation chromatography.
Appendix S2. Determination of kinetic parameters of recombinant OsGLYI-11.2.
Appendix S3. Confirmation of transgenic plants through Southern hybridization and RT-PCR.
Appendix S4. Leaf disc senescence assay.
Appendix S5.Na+/K+ estimation.
Appendix S6. Methylglyoxal (MG) estimation.
Appendix S7. Estimation of reduced glutathione.
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