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tpj12524-sup-0001-SupplementalMethods.docWord document57KMethods S1. Additional experimental procedures.
tpj12524-sup-0003-TableS1.pdfapplication/PDF38KTable S1. Sequences of proteins listed in Table 1.
tpj12524-sup-0004-TableS2.pdfapplication/PDF26KTable S2. Specific activities of purified S-RNase.
tpj12524-sup-0005-TableS3.pdfapplication/PDF150KTable S3. Pollination behaviors of the RNAi transgenic Petunia T1 progeny.
tpj12524-sup-0006-TableS4.pdfapplication/PDF31KTable S4. Primers used in this study.
tpj12524-sup-0007-TableS5.pdfapplication/PDF27KTable S5. ABCF genes in the apple genome.
tpj12524-sup-0008-FigS1.pdfapplication/PDF112KFigure S1. The architecture of plant ABC proteins.
tpj12524-sup-0009-FigS2.pdfapplication/PDF997KFigure S2. Sequence alignment of ABCF proteins in various apple cultivars and P. inflata.
tpj12524-sup-0010-FigS3.pdfapplication/PDF107KFigure S3. MdABCF transmembrane region prediction.
tpj12524-sup-0011-FigS4.pdfapplication/PDF88KFigure S4. RT-PCR analysis of MdABCF in various apple tissues.
tpj12524-sup-0012-FigS5.pdfapplication/PDF125KFigure S5. Establishing the time and concentration to be used for the as-ODN experiment.
tpj12524-sup-0013-FigS6.pdfapplication/PDF120KFigure S6. Effect of MdABCF down-regulation on S-RNase transportation.
tpj12524-sup-0014-FigS7.pdfapplication/PDF128KFigure S7. Influence of as-ODN treatment on pollen tubes.
tpj12524-sup-0015-FigS8.pdfapplication/PDF98KFigure S8. Influence of as-ODN on S-RNase transportation.
tpj12524-sup-0016-FigS9.pdfapplication/PDF99KFigure S9. Speed of uptake of FITC-labeled RNase I in pollen tubes.
tpj12524-sup-0017-FigS10.pdfapplication/PDF104KFigure S10. Immunofluorescence analysis of S-RNase uptake in apple pollen tubes.
tpj12524-sup-0018-FigS11.pdfapplication/PDF51KFigure S11. Interactions between PiABCF and S3L-RNase analyzed by the split-ubiquitin Y2H assay.
tpj12524-sup-0019-FigS12.pdfapplication/PDF111KFigure S12. Schematic representation of transgene constructs used for transformation experiments.
tpj12524-sup-0020-FigS13.pdfapplication/PDF43KFigure S13. RT-PCR analysis of PiABCF expression in RNAi transgenic Petunia T1 progeny.
tpj12524-sup-0021-FigS14.pdfapplication/PDF260KFigure S14. Interactions between apple MdABCF and S-RNases analyzed by the split-ubiquitin Y2H assay.
tpj12524-sup-0022-FigS15.pdfapplication/PDF65KFigure S15. Interactions between apple MdABCF and S-RNases analyzed by the split-ubiquitin Y2H assay.
tpj12524-sup-0023-FigS16.pdfapplication/PDF109KFigure S16. Self/non-self-S-RNase transport system analysis.
tpj12524-sup-0024-FigS17.pdfapplication/PDF96KFigure S17. Detection of a mixture of FITC-labeled S1-RNase and fluorescein isothiocyanate-4-ethyl rhodamine-labeled S2-RNase in S2 or S9 pollen.
tpj12524-sup-0025-FigS18.pdfapplication/PDF80KFigure S18. Analysis of treatment with as-ODN and various concentrations of S-RNase.
tpj12524-sup-0026-FigS19.pdfapplication/PDF132KFigure S19. Interaction between S-RNase and MdABCF-like proteins.
tpj12524-sup-0027-FigS20.pdfapplication/PDF115KFigure S20. Sequence analysis of non-S-RNase and RNase I.
tpj12524-sup-0028-FigS21.pdfapplication/PDF58KFigure S21. Hydrophobicity analysis of MdABCF by DNAman software.
tpj12524-sup-0029-FigS22.pdfapplication/PDF50KFigure S22. Northern blot analysis of PiABCF expression in transgene lines.
tpj12524-sup-0030-FigS23.pdfapplication/PDF308KFigure S23. Analysis of interactions between full-length MdABCF, MdABCF fragments TN2, T, N1T, N1 and N2 fragments and S1-RNase by pull-down assay.
tpj12524-sup-0031-FigS24.pdfapplication/PDF119KFigure S24. S-RNase transport analysis after treatment with as-ODN + LatA/taxol.
tpj12524-sup-0032-FigS25.pdfapplication/PDF98KFigure S25. Genotyping of RNAi progeny by PCR analysis.
tpj12524-sup-0033-FigS26.pdfapplication/PDF50KFigure S26. Positional analysis of S1-RNase in maize protoplasts.

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