The LORE1 insertion mutant resource (pages 306–317)
Anna Małolepszy, Terry Mun, Niels Sandal, Vikas Gupta, Manu Dubin, Dorian Urbański, Niraj Shah, Asger Bachmann, Eigo Fukai, Hideki Hirakawa, Satoshi Tabata, Marcin Nadzieja, Katharina Markmann, Junyi Su, Yosuke Umehara, Takashi Soyano, Akira Miyahara, Shusei Sato, Makoto Hayashi, Jens Stougaard and Stig U. Andersen
Version of Record online: 27 SEP 2016 | DOI: 10.1111/tpj.13243
Genome-wide insertional inactivation of genes has enabled functional genomics in increasing numbers of plant species. By activating an endogenous LTR retrotransoposon in the legume Lotus japonicus and annotating a very large number of insertion sites, nearly all active genes were targeted by activation of just a single LTR element. This was made possible due to the relaxed insertion site motif and preference for CHG-hypomethylated genes of the LTR element.