Integrating metabolomics and transcriptomics data to discover a biocatalyst that can generate the amine precursors for alkamide biosynthesis (pages 775–793)
Ludmila Rizhsky, Huanan Jin, Michael R. Shepard, Harry W. Scott, Alicen M. Teitgen, M. Ann Perera, Vandana Mhaske, Adarsh Jose, Xiaobin Zheng, Matt Crispin, Eve S. Wurtele, Dallas Jones, Manhoi Hur, Elsa Góngora-Castillo, C. Robin Buell, Robert E. Minto and Basil J. Nikolau
Version of Record online: 27 SEP 2016 | DOI: 10.1111/tpj.13295
The ethnobotanical history of Echinacea species is due to the ability to synthesize alkamide lipids, which have immune, insecticidal, and plant growth modulating properties. Here we used transcriptomicand metabolomic profiling to identify and characterize a broad-range, branched-chain amino acid (BCAA) decarboxylase in the biosynthetic pathway for alkamides.