The Plant Journal

Cover image for Vol. 89 Issue 1

January 2017

Volume 89, Issue 1

Pages 1–178

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. FEATURED ARTICLE
    4. ORIGINAL ARTICLES
    5. TECHNICAL ADVANCE
    1. Table of Contents (page 1)

      Version of Record online: 18 JAN 2017 | DOI: 10.1111/tpj.13353

  2. FEATURED ARTICLE

    1. Top of page
    2. Issue Information
    3. FEATURED ARTICLE
    4. ORIGINAL ARTICLES
    5. TECHNICAL ADVANCE
    1. You have full text access to this OnlineOpen article
      Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity (pages 3–14)

      Christian P. Craddock, Nicolette Adams, Johan T.M. Kroon, Fiona M. Bryant, Patrick J. Hussey, Smita Kurup and Peter J. Eastmond

      Version of Record online: 1 DEC 2016 | DOI: 10.1111/tpj.13321

      Significance Statement

      Coordinating endomembrane biogenesis as cells progress thorough the cell cycle is important. Here we uncover a CYCLIN-DEPENDENT KINASE A;1 regulatory mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis.

  3. ORIGINAL ARTICLES

    1. Top of page
    2. Issue Information
    3. FEATURED ARTICLE
    4. ORIGINAL ARTICLES
    5. TECHNICAL ADVANCE
    1. Changes in cytokinins are sufficient to alter developmental patterns of defense metabolites in Nicotiana attenuata (pages 15–30)

      Christoph Brütting, Martin Schäfer, Radomíra Vanková, Klaus Gase, Ian T. Baldwin and Stefan Meldau

      Version of Record online: 18 JAN 2017 | DOI: 10.1111/tpj.13316

      Significance statement

      Plant defense metabolites are regulated developmentally: young leaves are expected to have higher defense allocations than older leaves, but how development is coordinated with defense remains unknown. Here we genetically engineered transgenic plants to express cytokinin biosynthesis genes, using senescence-activated and chemically-inducible promoters. Genetically modifying the levels of different cytokinins in leaves was sufficient to alter ontogenic patterns of defense metabolites. We therefore concluded that developmental regulation of growth hormones plays central roles in establishing optimal patterns of defense allocations.

    2. DNA-binding and repressor function are prerequisites for the turnover of the tomato heat stress transcription factor HsfB1 (pages 31–44)

      Sascha Röth, Oliver Mirus, Daniela Bublak, Klaus-Dieter Scharf and Enrico Schleiff

      Version of Record online: 14 NOV 2016 | DOI: 10.1111/tpj.13317

      Significance Statement

      Transcription factor turnover is important in modulating gene transcription after developmental signals or environmental changes. Here we show that both the DNA-binding and repressor activity of the tomato heat stress transcription factor HsfB1 mediate its efficient turnover. We suggest that other transcription factors of the R/KLFGV-type might be regulated similarly.

    3. Electrostatic potentials of the S-locus F-box proteins contribute to the pollen S specificity in self-incompatibility in Petunia hybrida (pages 45–57)

      Junhui Li, Yue Zhang, Yanzhai Song, Hui Zhang, Jiangbo Fan, Qun Li, Dongfen Zhang and Yongbiao Xue

      Version of Record online: 14 NOV 2016 | DOI: 10.1111/tpj.13318

      Significance Statement

      Solanaceous self-incompatibility (SI) is controlled by a polymorphic S-locus encoding S-RNase and S-locus F-box (SLF) proteins to control the pistil and pollen expression of SI, respectively, but it remains unclear what physical forces contribute to their specific interactions. Here we show that the electrostatic potentials of SLFs act as a major force to contribute to the pollen S specificity, providing a mechanistic insight into the self/non-self discrimination between cytosolic proteins in angiosperms.

    4. Overexpression of a flower-specific aerolysin-like protein from the dioecious plant Rumex acetosa alters flower development and induces male sterility in transgenic tobacco (pages 58–72)

      Susana Manzano, Zoraida Megías, Cecilia Martínez, Alicia García, Encarnación Aguado, Tarik Chileh, Diego López-Alonso, Federico García-Maroto, Eduard Kejnovský, Jiří Široký, Zdeněk Kubát, Tereza Králová, Boris Vyskot and Manuel Jamilena

      Version of Record online: 24 NOV 2016 | DOI: 10.1111/tpj.13322

      Significance Statement

      In Rumex acetosa, FEM32 gene encodes for a protein, whose structure and domains resembles those of the bacterial pore-forming toxins of the aerolysin superfamily. The detailed characterization of the FEM32 molecular structure, and its overexpression in tobacco have suggested that it is probably involved in the arrest of stamen development and in pollen sterility.

    5. TMV induces RNA decay pathways to modulate gene silencing and disease symptoms (pages 73–84)

      Gabriela Conti, Diego Zavallo, Andrea L. Venturuzzi, Maria C. Rodriguez, Martin Crespi and Sebastian Asurmendi

      Version of Record online: 24 NOV 2016 | DOI: 10.1111/tpj.13323

      Significance Statement

      Plants combat viral infections by Post-Transcriptional Gene Silencing (PTGS) of viral transcripts. Most viruses have evolved viral suppressors of RNA silencing to attenuate or inhibit this process. Here, we show that tobacco plants infected with TMV or transgenic lines expressing the viral movement or coat proteins increase transcriptional levels of RNA decay genes, and propose that induction of RNA decay pathways interferes with PTGS. More importantly, this reprograming of host physiology interferes with endogenous physiological processes, thus producing symptoms.

    6. The Arabidopsis UDP-glycosyltransferases UGT79B2 and UGT79B3, contribute to cold, salt and drought stress tolerance via modulating anthocyanin accumulation (pages 85–103)

      Pan Li, Yan-Jie Li, Feng-Ju Zhang, Gui-Zhi Zhang, Xiao-Yi Jiang, Hui-Min Yu and Bing-Kai Hou

      Version of Record online: 1 DEC 2016 | DOI: 10.1111/tpj.13324

      Significance Statement

      The physiological roles of most UDP-glycosyltransferases (UGTs) are unknown. Here, we used overexpression and RNA interference or CRISPR-Cas9 strategies to show that two anthocyanin rhamnosyltransferases play crucial roles in enhancing tolerance to cold, salt and drought.

    7. Structural variability of plant photosystem II megacomplexes in thylakoid membranes (pages 104–111)

      Lukáš Nosek, Dmitry Semchonok, Egbert J. Boekema, Petr Ilík and Roman Kouřil

      Version of Record online: 1 DEC 2016 | DOI: 10.1111/tpj.13325

      Significance Statement

      Photosystem II (PSII) is a key protein complex involved in the light reactions of photosynthesis. Plant photosystem II forms multi-subunit pigment-protein supercomplexes that can associate into larger megacomplexes. Here we performed extensive structural analyses of isolated PSII megacomplexes to show that they are remarkably structurally variable. We propose that dynamic formation of different types of PSII megacomplexes can optimize use of absorbed light energy under variable environmental conditions.

    8. You have full text access to this OnlineOpen article
      PP2A-B′γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves (pages 112–127)

      Moona Rahikainen, Andrea Trotta, Sara Alegre, Jesús Pascual, Katariina Vuorinen, Kirk Overmyer, Barbara Moffatt, Stéphane Ravanel, Erich Glawischnig and Saijaliisa Kangasjärvi

      Version of Record online: 5 DEC 2016 | DOI: 10.1111/tpj.13326

      Significance Statement

      Glucosinolates (GSL), structurally diverse secondary compounds in crucifers, act as deterrents against aphids and microbial pathogens and consequently have commercial and ecological impacts. Transcriptional control of GSL biosynthesis and modification is understood, but post-translational modifications that determine structural variation has not been reported. It was known that protein phosphatase 2A (PP2A controls such stress-induced metabolic adjustments. Here we show that the PP2A regulatory subunit B’γ modulates cellular trans-methylation capacity and thus the formation of specifically methoxylated indole glucosinolates in Arabidopsis thaliana.

    9. Alternative splicing provides a proactive mechanism for the diurnal CONSTANS dynamics in Arabidopsis photoperiodic flowering (pages 128–140)

      Kyung-Eun Gil, Mi-Jeong Park, Hyo-Jun Lee, Young-Joon Park, Shin-Hee Han, Young-Ju Kwon, Pil Joon Seo, Jae-Hoon Jung and Chung-Mo Park

      Version of Record online: 5 DEC 2016 | DOI: 10.1111/tpj.13351

      Significance Statement

      Diverse molecular mechanisms are known to affect diurnal dynamics of the CONSTANS (CO) transcription factor in photoperiodic flowering, but it was unknown if CO itself affects its dynamics. Here, we show that CO undergoes alternative splicing, producing two protein isoforms, and that interactions between these two isoforms affect accessibility to E3 ubiquitin ligases for degradation, thus supporting that notion that CO self-regulates its own diurnal dynamics.

    10. You have full text access to this OnlineOpen article
      Isolation and characterization of mutants corresponding to the MENA, MENB, MENC and MENE enzymatic steps of 5′-monohydroxyphylloquinone biosynthesis in Chlamydomonas reinhardtii (pages 141–154)

      Barbara Emonds-Alt, Nadine Coosemans, Thomas Gerards, Claire Remacle and Pierre Cardol

      Version of Record online: 5 DEC 2016 | DOI: 10.1111/tpj.13352

      Significance Statement

      Phylloquinone is an essential electron carrier in photosystem I, but the entire biosynthetic pathway in Chlamydomonas was not known. Here we took advantage of the photosynthetic deficiency of a mend mutant to isolate new mutants in this pathway.

  4. TECHNICAL ADVANCE

    1. Top of page
    2. Issue Information
    3. FEATURED ARTICLE
    4. ORIGINAL ARTICLES
    5. TECHNICAL ADVANCE
    1. You have full text access to this OnlineOpen article
      Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit (pages 155–168)

      Jana Ordon, Johannes Gantner, Jan Kemna, Lennart Schwalgun, Maik Reschke, Jana Streubel, Jens Boch and Johannes Stuttmann

      Version of Record online: 14 NOV 2016 | DOI: 10.1111/tpj.13319

      Significance Statement

      In plants, genome editing has mostly been used to generate point mutations, but deletion alleles are often desirable. Here, we developed a toolkit for simple and efficient assembly of genome editing constructs and used it to generate deletions in both Nicotiana benthamiana and Arabidopsis. Small deletions (<100 bp) were recovered with relatively high frequencies in both species, while large deletions (up to 120 kb) were less frequent.

    2. You have full text access to this OnlineOpen article
      A robust, high-throughput method for computing maize ear, cob, and kernel attributes automatically from images (pages 169–178)

      Nathan D. Miller, Nicholas J. Haase, Jonghyun Lee, Shawn M. Kaeppler, Natalia de Leon and Edgar P. Spalding

      Version of Record online: 19 NOV 2016 | DOI: 10.1111/tpj.13320

      Significance Statement

      Maize researchers and breeders need precise and rapid ways to measure the key components of grain yield. Here we present custom algorithms that can compute such yield components automatically from digital images acquired with a flatbed document scanner, and demonstrate their utility with genetically diverse maize populations.

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