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tra12018-sup-0001-FigureS1.docWord document48KFigure S1: Mass spectrometry experiment to determine the number of installed BG molecules on CTB. Mass spectra of CTB labeled with BG at a molar ratio of 1:10 (upper panel) and of unlabeled CTB (lower panel).
tra12018-sup-0002-FigureS2.docWord document101KFigure S2: Cycloheximide (CHX) washout experiment to test the functionality of the drug CI-976. This assay is described in detail in [27]. Cells were transfected with HA-tagged caveolin-1 and cultured in medium containing 0.5 mm CHX to inhibit protein synthesis. After 3 h, when cells were attached to cover slips, CHX was washed away and cells were either incubated in medium containing 50 µm CI-976 or in medium without a drug. After a further 45 min, cells were fixed, stained with antibodies against HA and giantin and analyzed by confocal microscopy. Scale bars represent 10 µm.
tra12018-sup-0003-FigureS3.docWord document237KFigure S3: Full scans of the western blots and gels. A part of (A) is depicted in Figure 2E. A part of (B) is shown in Figure 4C, a part of (D) is shown in Figure 4G and a part of (E) is shown in Figure 5B. Unspecific bands are marked with an asterix throughout.
tra12018-sup-0004-Appendix S1.docxWord 2007 document22K Appendix S1: Considerations when using the SNAP-trap method.

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