tra12039-sup-0001-FigureS1.docxWord 2007 document101KFigure S1: Control experiments demonstrating functionality of Fur4 mutants and specificity of leflunomide treatment. A–C) Growth in presence or absence of 5-fluorouracil of fur4Δ strains containing either an empty vector (−) or plasmids expressing different versions of Fur4-GFP. Experiments shown in (A) and (C) used a different minimal medium than (B) (different auxotrophic selection). D) Control experiments testing that leflunomide does not induce downregulation of Mup1-GFP, (E) that uracil produced from cytosine in one cell does not induce downregulation of Fur4-GFP in another cell and (F) that Fur4(M96BPA)-GFP is able to efficiently import uracil from the growth medium.
tra12039-sup-0002-FigureS2.docxWord 2007 document129K Figure S2: LID-loop interactions in the ground state of Mhp1. ligplot of the Mhp1 LID based on the crystal structure of the outward-open conformation (ground state, 2JLN).
tra12039-sup-0003-FigureS3.docxWord 2007 document124K Figure S3: LID-loop interactions in the substrate-bound state of Mhp1. ligplot of the Mhp1 LID based on the crystal structure of the inward-occluded conformation (substrate-bound state, 2X79).

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