tra12125-sup-0001-FigureS1.docWord document883KFigure S1: End3 expression levels are reduced in E3R mutants. Immunoblots of mid-log cultures grown at 30°C, or shifted to 37°C for 1 h. Cells were TCA precipitated and extracts resolved by SDS–PAGE, transferred to nitrocellulose, and probed with anti-End3 or anti-Actin antibody. Strains in this assay were either wildtype with empty vector or end3Δ with the indicated plasmid.
tra12125-sup-0002-FigureS2.docWord document1759KFigure S2: Images of Mup1-pHluorin uptake in End3 repeat mutant strains. Representative images of the quantified changes in Mup1-pHluorin fluorescence intensity after methionine addition (from the experiment shown in Figure 7). For each image, maximum and minimum intensities were normalized to the same level to reflect changes in the relative Mup1 fluorescence over time. Scale bar, 10 microns.
tra12125-sup-0003-TableS1.docWord document112KTable S1: Novel yeast two-hybrid binding partners with Pan1's central coiled-coil

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