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tra12167-sup-0001-FigureS1.docWord document637KFigure S1. Golgi ribbon fragmentation induced by COG3 depletion as shown at the individual cell level was suppressed by BicD depletion. HeLa cells stably expressing GalNAcT2-GFP were incubated with siControl (A), siCOG3 (B), siBicD1 (D), siBicD1 + siCOG3 (E), siBicD2 (G) and siBicD2 + siCOG3 (H) RNAs. C, F and I) COG3 antibody staining at the individual cell level that depletion of the protein was extensive under all conditions and suppression was achieved in cells in little, if any, COG3 staining was apparent: siCOG3 (C), siBicD1 + siCOG3 (F) and siBicD2 + siCOG3 (I), respectively. Total siRNA incubation time was 96 h.
tra12167-sup-0002-FigureS2.docWord document489KFigure S2. Depletion of cytoplasmic dynein heavy chain 1 (DHC1) produces enhanced Golgi ribbon dispersal/fragmentation in COG3- or ZW10-depleted cells. HeLa cells stably expressing GalNAcT2-GFP were treated with siControl (A) or siCOG3 (B) and siZW10 (C) and then fixed. Alternatively, cells were incubated with siDynein (D), siDynein + siCOG3 (E) and siDynein + siZW10 (F) and then fixed. Epistatic double knock of siDynein and siCOG3 or ZW10 resulted in epistatic enhancement of Golgi ribbon fragmentation (E and F). Total siRNA incubation time was 96 h.

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