The CryoCapsule: Simplifying Correlative Light to Electron Microscopy (pages 700–716)
Xavier Heiligenstein, Jérôme Heiligenstein, Cédric Delevoye, Ilse Hurbain, Sabine Bardin, Perrine Paul-Gilloteaux, Lucie Sengmanivong, Gilles Régnier, Jean Salamero, Claude Antony and Graca Raposo
Version of Record online: 24 MAR 2014 | DOI: 10.1111/tra.12164
Correlating complementary multiple scale images of the same object is a straightforward means to decipher biological processes. Light microscopy and electron microscopy are the most commonly used imaging techniques, yet despite their complementarity, the experimental procedures available to correlate them are technically complex. We designed and manufactured a new device adapted to many biological specimens, the CryoCapsule, that simplifies the multiple sample preparation steps, which at present separate live cell fluorescence imaging from contextual high-resolution electron microscopy.