Spray-dried plasma and fresh frozen plasma modulate permeability and inflammation in vitro in vascular endothelial cells

Authors

  • K. Wataha,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • T. Menge,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • X. Deng,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • A. Shah,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • A. Bode,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • J.B. Holcomb,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • D. Potter,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • R. Kozar,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • P.C. Spinella,

    1. Blood Systems Research Institute, University of California San Francisco, San Francisco, California
    2. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    3. Entegrion, Inc., Research Triangle Park, North Carolina
    4. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    5. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    6. United States Army Institute of Surgical Research, San Antonio, Texas
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  • S. Pati

    Corresponding author
    1. Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    2. Entegrion, Inc., Research Triangle Park, North Carolina
    3. The Department of Surgery and Center for Translational Injury Research, University of Texas, Health Sciences Center (UTHSC), Houston, Texas
    4. Washington University in St Louis, Division of Critical Care, Department of Pediatrics, St Louis, Missouri
    5. United States Army Institute of Surgical Research, San Antonio, Texas
    • Blood Systems Research Institute, University of California San Francisco, San Francisco, California
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  • This study was supported in part by research funding from Entegrion to SP. Entegrion is a recipient of a grant from the Office of Naval Research under Contract No. N00014-10-C-0333 that supports this study.

Address reprint requests to: S. Pati, Blood Systems Research Institute, 270 Masonic Ave., San Francisco, CA 94118; e-mail: spati@bloodsystems.org.

Abstract

Background

After major traumatic injury, patients often require multiple transfusions of fresh frozen plasma (FFP) to correct coagulopathy and to reduce bleeding. A spray-dried plasma (SDP) product has several logistical benefits over FFP use in trauma patients with coagulopathy. These benefits include ease of transport, stability at room temperature, and rapid reconstitution for infusion. Our past work suggests that FFP promotes endothelial stability by inhibiting endothelial permeability.

Study Design and Methods

The main goal of this project is to determine if solvent-detergent-treated SDP is equivalent to FFP in inhibiting vascular endothelial cell (EC) permeability and inflammation in vitro. Furthermore, this study aimed to determine if solvent-detergent treatment and spray drying of plasma alters the protective effects of FFP on EC function. The five groups tested in our studies are the following: 1) fresh frozen-thawed plasma (FFP); 2) solvent-detergent-treated FFP; 3) solvent-detergent-treated SDP; 4) lactated Ringer's solution; and 5) Hextend.

Results

This study demonstrates that in vitro SDP and FFP equivalently inhibit vascular EC permeability, EC adherens junction breakdown, and endothelial white blood cell binding, an effect that is independent of changes in Vascular Cell Adhesion Molecule 1, Intracellular Adhesion Molecule 1, or E-selectin expression on ECs. Solvent-detergent treatment of FFP does not alter the protective effects of FFP on endothelial cell function in vitro.

Conclusion

These data suggest the equivalence of FFP and SDP on modulation of endothelial function and inflammation in vitro.

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