Fig. S1. Comparison of anti-HPA-1a alloantibody semi-quantification using MAIPA and SPR. Anti-HPA-1a alloantibodies were semi-quantified in maternal sera from Group I (n = 6) using MAIPA and SPR against an in-house standard serum containing anti-HPA-1a alloantibodies (assigned potency of 1000 AU/mL). Reference serum was initially diluted to 31 AU/mL. The standard curve was constructed with a non-linear regression, by plotting OD in MAIPA and RU at the end of the association step (B350, left panel) and at the end of the disassociation step (B700, right panel) in SPR for six dilutions of the reference.

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