Both laboratories contributed equally to the study.
IMMUNE HEMATOLOGIC DISEASE
The implementation of surface plasmon resonance technique in monitoring pregnancies with expected fetal and neonatal alloimmune thrombocytopenia
Article first published online: 24 DEC 2012
© 2012 American Association of Blood Banks
Volume 53, Issue 9, pages 2078–2085, September 2013
How to Cite
Bakchoul, T., Bertrand, G., Krautwurst, A., Kroll, H., Bein, G., Sachs, U. J., Santoso, S. and Kaplan, C. (2013), The implementation of surface plasmon resonance technique in monitoring pregnancies with expected fetal and neonatal alloimmune thrombocytopenia. Transfusion, 53: 2078–2085. doi: 10.1111/trf.12051
This work was supported by a grant for TB from the University Hospital Giessen and Marburg GmbH.
- Issue published online: 9 SEP 2013
- Article first published online: 24 DEC 2012
- Manuscript Accepted: 30 OCT 2012
- Manuscript Revised: 9 OCT 2012
- Manuscript Received: 8 JUN 2012
- University Hospital Giessen and Marburg GmbH
Fig. S1. Comparison of anti-HPA-1a alloantibody semi-quantification using MAIPA and SPR. Anti-HPA-1a alloantibodies were semi-quantified in maternal sera from Group I (n = 6) using MAIPA and SPR against an in-house standard serum containing anti-HPA-1a alloantibodies (assigned potency of 1000 AU/mL). Reference serum was initially diluted to 31 AU/mL. The standard curve was constructed with a non-linear regression, by plotting OD in MAIPA and RU at the end of the association step (B350, left panel) and at the end of the disassociation step (B700, right panel) in SPR for six dilutions of the reference.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.