Proteomic analysis of platelets treated with gamma irradiation versus a commercial photochemical pathogen reduction technology
Article first published online: 10 JAN 2013
© 2013 American Association of Blood Banks
Volume 53, Issue 8, pages 1808–1820, August 2013
How to Cite
Marrocco, C., D'Alessandro, A., Girelli, G. and Zolla, L. (2013), Proteomic analysis of platelets treated with gamma irradiation versus a commercial photochemical pathogen reduction technology. Transfusion, 53: 1808–1820. doi: 10.1111/trf.12060
- Issue published online: 8 AUG 2013
- Article first published online: 10 JAN 2013
- Manuscript Accepted: 12 OCT 2012
- Manuscript Revised: 20 SEP 2012
- Manuscript Received: 7 AUG 2012
- Italian Ministry of Health
- Italian National Blood Centre (Centro Nazionale Sangue–CNS, Istituto Superiore Sanità, Rome, Italy)
- Interuniversity Consortium for Biotechnology (CIB)
Several strategies are currently being tested to reduce the risk of pathogen transmission associated with platelet (PLT) transfusion. Within the framework of the Italian Platelet Technology Assessment Study, we investigated the variations of the protein profiles (proteomics) of apheresis PLT concentrates (PCs) upon treatment with riboflavin and ultraviolet (UV) light (Mirasol; 6.24 J/mL; 280-400 nm).
Study Design and Methods
Control, gamma-irradiated, and Mirasol-treated apheresis PCs were assayed on Days 1 and 5 of storage by means of gel-based analytical approaches (two-dimensional gel electrophoresis) and mass spectrometry–based identification of significant (p < 0.05 analysis of variance) differential proteins. Supernatants were then assayed for metabolism and oxidative stress-related metabolites through multiple reaction monitoring mass spectrometry.
Only a handful of modifications could be observed in the PLT proteome profiles in response to the Mirasol treatment, which included proteins involved in oxidative stress responses, PLT metabolism, and activation. Results confirmed increased metabolic rate and oxidative stress in the supernatants of treated PLTs (both gamma irradiated and Mirasol treated).
From this investigation, it emerges that, from a proteomics standpoint, gamma irradiation results in the acceleration of PLT storage lesions and the Mirasol treatment only moderately exacerbates these phenomena.