These authors contributed equally.
DONOR INFECTIOUS DISEASE TESTING
Routine bacterial screening of apheresis platelets on Day 4 using a rapid test: a 4-year single-center experience
Article first published online: 24 JAN 2013
© 2013 American Association of Blood Banks
Volume 53, Issue 10, pages 2307–2313, October 2013
How to Cite
Dunbar, N. M., Kreuter, J. D., Marx-Wood, C. R., Dumont, L. J. and Szczepiorkowski, Z. M. (2013), Routine bacterial screening of apheresis platelets on Day 4 using a rapid test: a 4-year single-center experience. Transfusion, 53: 2307–2313. doi: 10.1111/trf.12083
- Issue published online: 4 OCT 2013
- Article first published online: 24 JAN 2013
- Manuscript Revised: 21 NOV 2012
- Manuscript Accepted: 21 NOV 2012
- Manuscript Received: 18 SEP 2012
The platelet (PLT) Pan Genera Detection test (PGD) is a rapid bacterial detection system used to screen PLTs for bacterial contamination. We report a single center 46-month experience with secondary screening of apheresis PLTs by PGD testing.
Study Design and Methods
Existing testing records of apheresis PLTs screened by PGD from July 2008 to April 2012 were reviewed. All PLT units were initially screened by routine postcollection culture methods. Secondary screening using PGD was performed for indated PLTs on PLT storage Day 4 and for outdated PLTs on Day 8.
A total of 8535 apheresis PLTs were available in inventory during the study period. Of these, 5030 (58.9%) were dispensed and transfused before PGD testing and 3505 (41.1%) underwent PGD testing on Day 4. Twenty-five units tested on Day 4 were PGD initial reactive (0.71%). All were confirmed to be false positive by repeat PGD testing in triplicate (n = 20) or by confirmatory culture (n = 5). An additional 364 units that were PGD nonreactive on Day 4 were approved for transfusion on Day 6 or Day 7 due to urgent clinical need. A total of 371 outdated units underwent repeat PGD testing before discard on Day 8; all were nonreactive.
Secondary PGD testing of culture-screened apheresis PLTs results in low yield in a medium-sized transfusion service. Use of PGD testing on Day 4 may allow for extension of the apheresis PLT shelf life to Day 7 for hospitals that face supply constraints.