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Measurements of human herpesvirus 8 viral load in blood before and after leukoreduction filtration

Authors

  • Sheila C. Dollard,

    Corresponding author
    1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    2. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
    • Centers for Disease Control and Prevention, Atlanta, Georgia
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  • John D. Roback,

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • Clifford Gunthel,

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • Minal M. Amin,

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • Sheilagh Barclay,

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • Ericka Patrick,

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • Matthew J. Kuehnert

    1. Centers for Disease Control and Prevention, Atlanta, Georgia
    2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
    3. HIV/AIDS Clinical Trials Unit, Emory University School of Medicine, Atlanta, Georgia
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  • The findings and conclusions in this report are those of the authors and do not necessarily represent the official views of the Centers for Disease Control and Prevention.
  • This study was funded by the Centers for Disease Control and Prevention.

Address correspondence to: Sheila C. Dollard, 1600 Clifton Road NE, Mailstop G18, Atlanta, GA 30333; e-mail: sdollard@cdc.gov.

Abstract

Background

Human herpesvirus 8 (HHV-8) is likely transmitted through blood transfusion in high-prevalence areas. The efficacy of leukoreduction filtration for reducing HHV-8 in blood has not been reported.

Study Design and Methods

Blood was drawn from 45 human immunodeficiency virus–positive men either with Kaposi's sarcoma (KS; n = 21) or without KS (n = 24) and subject to leukoreduction filtration. HHV-8 viral load was measured in plasma and in blood before and after filtration.

Results

Twelve subjects, all with KS, had detectable HHV-8 viremia before filtration with viral loads of 102 to 105 copies/mL (mean, 3 × 104 copies/mL). After filtration, seven of 12 subjects no longer had detectable HHV-8 in their blood, and five of 12 subjects had detectable HHV-8 that was 90% reduced on average from prefiltration levels. The presence of HHV-8 in the blood after filtration was strongly associated with prefiltration viral loads greater than 1000 copies/mL and the presence of cell-free virus in plasma. None of the subjects without KS had detectable levels of HHV-8 virus in blood before or after filtration.

Conclusion

Cell-associated HHV-8 appeared to be effectively removed by leukoreduction filtration. Cell-free HHV-8 was present in 42% of subjects as 1% to 20% of the total virus which was not removed by filtration.

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