Co-first authors: QXA, CYL, and LJX contributed equally to this work.
BLOOD GROUP GENOMICS
High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array
Version of Record online: 3 APR 2013
© 2013 American Association of Blood Banks
Volume 53, Issue 11, pages 2722–2728, November 2013
How to Cite
An, Q.-X., Li, C.-Y., Xu, L.-J., Zhang, X.-Q., Bai, Y.-J., Shao, Z.-J. and Zhang, W. (2013), High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array. Transfusion, 53: 2722–2728. doi: 10.1111/trf.12164
This research was supported by the National Natural Science Foundation of China (No. 30571675) and the Natural Science Foundation of Shaanxi Province, China (2011 K12-05-15).
- Issue online: 13 NOV 2013
- Version of Record online: 3 APR 2013
- Manuscript Accepted: 10 JAN 2013
- Manuscript Revised: 15 DEC 2012
- Manuscript Received: 8 JUN 2012
- National Natural Science Foundation of China. Grant Number: 30571675
- Natural Science Foundation of Shaanxi Province, China. Grant Number: 2011 K12-05-15
Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high-throughput simultaneous detection of HPA-1 to -16, and the accuracy of many methods needs to be further enhanced.
Study Design and Methods
We have developed a new HPA-genotyping method for simultaneous detection of HPA-1 to -16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence-based typing (PCR-SBT), which is considered the gold standard.
All 216 samples were successfully genotyped for HPA-1 to -16 using both our method and PCR-SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR-SBT.
Our method provides accurate, high-throughput, and simultaneous genotyping of HPA-1 to -16 and will serve as the foundation for large-scale clinical genotyping of HPAs and for the establishment of an HPA-typed PLT donor registry.