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High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array

Authors

  • Qun-Xing An,

    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
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  • Cui-Ying Li,

    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
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  • Li-Juan Xu,

    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
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  • Xian-Qing Zhang,

    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
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  • Yan-Jun Bai,

    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
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  • Zhong-Jun Shao,

    Corresponding author
    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
    • Address reprint requests to: Zhong-Jun Shao, Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China; e-mail: zhjshao@hotmail.com or Wei Zhang, State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an 710033, China; e-mail: zhangw90@fmmu.edu.cn.

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  • Wei Zhang

    Corresponding author
    1. Department of Blood Transfusion, Xijing Hospital, Xi'an, China
    2. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China
    3. State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, China
    4. Department of Blood Transfusion, Chengdu Military General Hospital, Chengdu, China
    5. Department of Blood Transfusion, 307 Hospital of PLA, Beijing, China
    • Address reprint requests to: Zhong-Jun Shao, Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China; e-mail: zhjshao@hotmail.com or Wei Zhang, State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an 710033, China; e-mail: zhangw90@fmmu.edu.cn.

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  • Co-first authors: QXA, CYL, and LJX contributed equally to this work.
  • This research was supported by the National Natural Science Foundation of China (No. 30571675) and the Natural Science Foundation of Shaanxi Province, China (2011 K12-05-15).

Abstract

Background

Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high-throughput simultaneous detection of HPA-1 to -16, and the accuracy of many methods needs to be further enhanced.

Study Design and Methods

We have developed a new HPA-genotyping method for simultaneous detection of HPA-1 to -16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence-based typing (PCR-SBT), which is considered the gold standard.

Results

All 216 samples were successfully genotyped for HPA-1 to -16 using both our method and PCR-SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR-SBT.

Conclusion

Our method provides accurate, high-throughput, and simultaneous genotyping of HPA-1 to -16 and will serve as the foundation for large-scale clinical genotyping of HPAs and for the establishment of an HPA-typed PLT donor registry.

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