Fig. S1. The RHD exon 8 marker displays an alternative pattern in some compound heterozygous samples by RHD-QMPSF analysis: (A) wild-type homozygous, (B) compound heterozygous [RHDΨ + DAU-4], and (C) compound heterozygous [RHDΨ + weak D, type 4.2.3]. Scale (base-pairs (bps)) is indicated above the schemes; e8 and e10 respectively refer to as the markers for exons 8 and 10; * refers to as the exon 8 marker plus the 4-bp insertion; number of the respective markers are indicated below the schemes.


Fig. S2. Possible combinations of variant D alleles in a donor presenting with D ambiguity. To explain the molecular pattern observed by QMPSF analyses, five different scenarios involving one or two D-CE hybrid genes are suggested based on RhesusBase version 2.0 ( Black squares: RHD-derived exons; white squares: RHCE-derived exons.


Table S1. Genotypes of the six compound heterozygous samples carrying a D-negative allele and results of the QMPSF analyses.

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