Supported by the Etablissement Français du Sang (EFS)–Bretagne and the Institut National de la Santé et de la Recherche Médicale (INSERM), France.
A convenient qualitative and quantitative method to investigate RHD-RHCE hybrid genes
Article first published online: 3 APR 2013
© 2013 American Association of Blood Banks
Special Issue: Twenty Years since the Cloning of the Blood Group Genes
Volume 53, Issue 11pt2, pages 2974–2982, November 2013
How to Cite
Fichou, Y., Le Maréchal, C., Bryckaert, L., Dupont, I., Jamet, D., Chen, J.-M. and Férec, C. (2013), A convenient qualitative and quantitative method to investigate RHD-RHCE hybrid genes. Transfusion, 53: 2974–2982. doi: 10.1111/trf.12179
- Issue published online: 12 NOV 2013
- Article first published online: 3 APR 2013
- Manuscript Accepted: 10 FEB 2013
- Manuscript Revised: 24 JAN 2013
- Manuscript Received: 5 DEC 2012
- Etablissement Français du Sang (EFS)–Bretagne and the Institut National de la Santé et de la Recherche Médicale (INSERM)
Fig. S1. The RHD exon 8 marker displays an alternative pattern in some compound heterozygous samples by RHD-QMPSF analysis: (A) wild-type homozygous, (B) compound heterozygous [RHDΨ + DAU-4], and (C) compound heterozygous [RHDΨ + weak D, type 4.2.3]. Scale (base-pairs (bps)) is indicated above the schemes; e8 and e10 respectively refer to as the markers for exons 8 and 10; * refers to as the exon 8 marker plus the 4-bp insertion; number of the respective markers are indicated below the schemes.
Fig. S2. Possible combinations of variant D alleles in a donor presenting with D ambiguity. To explain the molecular pattern observed by QMPSF analyses, five different scenarios involving one or two D-CE hybrid genes are suggested based on RhesusBase version 2.0 (http://www.uni-ulm.de/~fwagner/RH/RB2/). Black squares: RHD-derived exons; white squares: RHCE-derived exons.
Table S1. Genotypes of the six compound heterozygous samples carrying a D-negative allele and results of the QMPSF analyses.
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