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Appendix: Testing algorithms and standardization details.

Table S1. Analytical sensitivity of Ultrio and Ultrio Plus as determined by parallel probit analysis on different dose ranges of WHO standard dilution panels.

Table S2. Quantification of primary HBV-DNA genotype A, HCV genotype 1 and HIV-1 subtype B standards (VQC-Sanquin, Amsterdam, the Netherlands) in cps/mL using different viral load assays from 1997 until 2008.

Table S3A. Calibration of Sanquin-VQC HBV-DNA genotype A standard (quantified in bDNA 3.0 cps/mL) against (i) the WHO genotype A (97/746 and 97/750) standards calibrated at 50.000 International Units (IUs) per ampoule by Saldanha et al.,2,5 (ii) the Eurohep genotype A standard quantified at 2.7 × 10E9 cps/mL by Heerman et al. (ref 13, main article) and (iii) Chimpanzee genotype A and C plasmas with known infectivity titers and quantified in cps/mL in RocheTaqMan assay by Komiya et al. (ref 15, main article).

Table S3B. Calibration of the Sanquin-VQC HCV-RNA genotype 1 standard (quantified in bDNA 3.0 cps/mL) against (i) the WHO genotype 1 (96/790, 96/978 and 06/100) standards calibrated at 50.000, 50.000 and 77.441 IUs per ampoule by Saldanha et al.1 and Baylis et al.6 and (iii) a chimpanzee genotype 1b plasma with a known infectivity titer and quantified in cps/mL in RocheTaqMan assay by Katayama et al. (ref 16, main article).

Table S3C. Calibration of VQC-Sanquin HIV-RNA subtype B standard on the first (97/656) and second (97/650) WHO HIV-1 RNA subtype B standards (containing 100,000 and 363,078 IU per ampoule respectively)3,4 as calculated from individual quantitative assays on standard dilutions with five methods as reported by the laboratories participating in the first WHO collaborative study.3

Table S4A. Calibration of secondary HBV-DNA standards of different genotypes against the primary VQC-Sanquin HBV genotype A standard containing 2.15E + 09 cps/mL according to quantification in 28 bDNA 3.0 assays (Supplemental Table 2).

Table S4B. Calibration of secondary HCV-RNA standards of different genotypes against the primary VQC-Sanquin HCV genotype 1a/b standard containing 6.30E + 06 cps/mL according to quantification in 27 bDNA 3.0 assays (Supplemental Table 2).

Table S4C. Calibration of secondary HIV-1 RNA standards of different subtypes against the primary VQC-Sanquin HIV-1 subtype B standard containing 1.05E + 08 cps/mL according to quantification in 58 bDNA 3.0 assays (Supplemental Table 2).

Table S5A. Response rates of Ultrio and Ultrio Plus on BioQControl HBV-DNA genotype standard dilution panels.

Table S5B. Response rates of Ultrio and Ultrio Plus on BioQControl HCV-RNA genotype standard dilution panels.

Table S5C. Response rates of Ultrio and Ultrio Plus on BioQControl HIV-1 RNA subtype standard dilution panels.

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