This study was supported by Grant N N404 188036 from the Polish Committee for Scientific Research.
RHD variants in Polish blood donors routinely typed as D–
Version of Record online: 1 MAY 2013
© 2013 American Association of Blood Banks
Special Issue: Twenty Years since the Cloning of the Blood Group Genes
Volume 53, Issue 11pt2, pages 2945–2953, November 2013
How to Cite
Orzińska, A., Guz, K., Polin, H., Pelc-Kłopotowska, M., Bednarz, J., Gieleżyńska, A., Śliwa, B., Kowalewska, M., Pawłowska, E., Włodarczyk, B., Malaga, Alicja Żmudzin, M., Krzemienowska, M., Srivastava, K., Michalewska, B., Gabriel, C., Flegel, W. A. and Brojer, E. (2013), RHD variants in Polish blood donors routinely typed as D–. Transfusion, 53: 2945–2953. doi: 10.1111/trf.12230
- Issue online: 12 NOV 2013
- Version of Record online: 1 MAY 2013
- Manuscript Accepted: 10 FEB 2013
- Manuscript Revised: 10 JAN 2013
- Manuscript Received: 3 OCT 2012
- Polish Committee for Scientific Research. Grant Number: N N404 188036
Blood donors exhibiting a weak D or DEL phenotypical expression may be mistyped D– by standard serology hence permitting incompatible transfusion to D– recipients. Molecular methods may overcome these technical limits. Our aim was to estimate the frequency of RHD alleles among the apparently D– Polish donor population and to characterize its molecular background.
Study Design and Methods
Plasma pools collected from 31,200 consecutive Polish donors typed as D– were tested by real-time polymerase chain reaction (PCR) for the presence of RHD-specific markers located in Intron 4 and Exons 7 and 10. RHD+ individuals were characterized by PCR or cDNA sequencing and serology.
Plasma cross-pool strategy revealed 63 RHD+ donors harboring RHD*01N.03 (n = 17), RHD*15 (n = 12), RHD*11 (n = 7), RHD*DEL8 (n = 3), RHD*01W.2 (n = 3), RHD-CE(10) (n = 3), RHD*01W.3, RHD*01W.9, RHD*01N.05, RHD*01N.07, RHD*01N.23, and RHD(IVS1-29G>C) and two novel alleles, RHD*(767C>G) (n = 3) and RHD*(1029C>A). Among 47 cases available for serology, 27 were shown to express the D antigen
1) Plasma cross-pool strategy is a reliable and cost-effective tool for RHD screening. 2) Only 0.2% of D– Polish donors carry some fragments of the RHD gene; all of them were C or E+. 3) Almost 60% of the detected RHD alleles may be potentially immunogenic when transfused to a D– recipient.