Segments from red blood cell units should not be used for quality testing
Article first published online: 9 JUL 2013
© 2013 American Association of Blood Banks
Volume 54, Issue 2, pages 451–455, February 2014
How to Cite
Kurach, J. D.R., Hansen, A. L., Turner, T. R., Jenkins, C. and Acker, J. P. (2014), Segments from red blood cell units should not be used for quality testing. Transfusion, 54: 451–455. doi: 10.1111/trf.12303
- Issue published online: 11 FEB 2014
- Article first published online: 9 JUL 2013
- Manuscript Revised: 2 MAY 2013
- Manuscript Accepted: 2 MAY 2013
- Manuscript Received: 1 APR 2013
Nondestructive testing of blood components could permit in-process quality control and reduce discards. Tubing segments, generated during red blood cell (RBC) component production, were tested to determine their suitability as a sample source for quality testing.
Study Design and Methods
Leukoreduced RBC components were produced from whole blood (WB) by two different methods: WB filtration and buffy coat (BC). Components and their corresponding segments were tested on Days 5 and 42 of hypothermic storage (HS) for spun hematocrit (Hct), hemoglobin (Hb) content, percentage hemolysis, hematologic indices, and adenosine triphosphate concentration to determine whether segment quality represents unit quality.
Segment samples overestimated hemolysis on Days 5 and 42 of HS in both BC- and WB filtration–produced RBCs (p < 0.001 for all). Hct and Hb levels in the segments were also significantly different from the units at both time points for both production methods (p < 0.001 for all). Indeed, for all variables tested different results were obtained from segment and unit samples, and these differences were not consistent across production methods.
The quality of samples from tubing segments is not representative of the quality of the corresponding RBC unit. Segments are not suitable surrogates with which to assess RBC quality.