The Australian government fully funded the Australian Red Cross Blood Service for the provision of blood products and services to the Australian community. This work was funded in part by NIH Grant 1R01 HL095470-01A1.
In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study
Version of Record online: 22 JUL 2013
© 2013 Australian Red Cross. Transfusion © 2013 American Association of Blood Banks
Volume 54, Issue 3, pages 560–568, March 2014
How to Cite
Sparrow, R. L., Sran, A., Healey, G., Veale, M. F. and Norris, P. J. (2014), In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study. Transfusion, 54: 560–568. doi: 10.1111/trf.12344
- Issue online: 11 MAR 2014
- Version of Record online: 22 JUL 2013
- Manuscript Accepted: 29 MAY 2013
- Manuscript Revised: 17 MAY 2013
- Manuscript Received: 15 MAR 2013
- NIH. Grant Number: HL095470-01A1
Saline-adenine-glucose-mannitol (SAGM) and a variant solution, AS-1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS-1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS-1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Compared to SAGM RBCs, AS-1 RBCs had lower hemolysis (p < 0.04), lower GPA+ MPs (p < 0.03), and lower PS+ MPs (p < 0.03) from Day 14 onward. AS-1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS-1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02).
SAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS-1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS-1 RBCs after transfusion remains to be determined.