This work was supported by grants from the French National Blood Service–EFS (Grant APR), France; the Association for Research in Transfusion (ART), Paris, France; the Agence Nationale de la Sécurité et du Médicament et des produits de santé (ANSM–AAP-2012-011, Reference 2012S055); and the Association “Les Amis de Rémi,” Savigneux, France.
Immune-reactive soluble OX40 ligand, soluble CD40 ligand, and interleukin-27 are simultaneously oversecreted in platelet components associated with acute transfusion reactions
Article first published online: 14 AUG 2013
© 2013 American Association of Blood Banks
Volume 54, Issue 3, pages 613–625, March 2014
How to Cite
Hamzeh-Cognasse, H., Damien, P., Nguyen, K. A., Arthaud, C.-A., Eyraud, M.-A., Chavarin, P., Absi, L., Osselaer, J.-C., Pozzetto, B., Cognasse, F. and Garraud, O. (2014), Immune-reactive soluble OX40 ligand, soluble CD40 ligand, and interleukin-27 are simultaneously oversecreted in platelet components associated with acute transfusion reactions. Transfusion, 54: 613–625. doi: 10.1111/trf.12378
HHC and FC designed and performed the study, analyzed the data, and wrote the paper; KAN, PD, CAA, MAE, PC, BP, and LA analyzed the data; JCO produced and analyzed the data; and OG coordinated the study, cowrote the manuscript, and supervised the entire project.
- Issue published online: 11 MAR 2014
- Article first published online: 14 AUG 2013
- Manuscript Accepted: 10 JUN 2013
- Manuscript Received: 8 JUN 2013
- French National Blood Service–EFS
- Association for Research in Transfusion
- Agence Nationale de la Sécurité et du Médicament et des produits de santé. Grant Number: ANSM–AAP-2012-011
- Association “Les Amis de Rémi,” Savigneux, France
Figure S1. Representative B and T cell purity (negative selection). PBMNC) were isolated from blood of healthy donors. Cells were stained with 7-aminoactinomycin D (7-AAD) (viability dye), anti-CD45-allophycocyanin (APC), anti-CD3-fluorescein isothiocyanate (FITC) (T cell marker) and anti-CD19-allophycocyanin (APC) (B cell marker) for 15 minutes at room temperature in the dark. Data are representative of 10 independent experiments.
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