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Fig. S1. Allelic discrimination assays. Typical HPA-21 (A) and HPA-12 (B) results shown for 50 fathers of unresolved NAIT cases. Signal intensities of Cy5 (HPA-21bw or HPA-12bw) versus FAM (HPA-21a or HPA-12bw) fluorophores are plotted. No template controls are denoted by grey diamonds. Homozygous and heterozygous samples are denoted with black diamonds and triangles respectively. Unknowns that tested positive in the assay are denoted with grey circles. Each unknown that typed at HPA-21a/bw or HPA-12a/bw was verified with sequence analysis.

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