The views expressed do not necessarily represent the view of the National Institutes of Health, the Department of Health and Human Services, or the U.S. Federal Government.
A new blood group antigen is defined by anti-CD59, detected in a CD59-deficient patient
Article first published online: 3 JAN 2014
© 2014 AABB
How to Cite
Anliker, M., von Zabern, I., Höchsmann, B., Kyrieleis, H., Dohna-Schwake, C., Flegel, W. A., Schrezenmeier, H. and Weinstock, C. (2014), A new blood group antigen is defined by anti-CD59, detected in a CD59-deficient patient. Transfusion. doi: 10.1111/trf.12531
- Article first published online: 3 JAN 2014
- Manuscript Accepted: 31 OCT 2013
- Manuscript Revised: 10 OCT 2013
- Manuscript Received: 15 JUL 2013
CD59 is a cell surface glycoprotein of approximately 20 kDa limiting the lytic activity of the terminal complement complex C5b-9. Although CD59 is known as a red blood cell (RBC) antigen defined by monoclonal antibodies, it so far has not been identified as a blood group antigen, since the description of a human alloantibody was missing. In this study we show the presence of an anti-CD59 in a patient affected by a homozygous CD59 deficiency.
Study Design and Methods
RBC CD59 and CD55 were determined by flow cytometry or by the column agglutination technique using monoclonal antisera. Commercially available His-tagged recombinant soluble CD59 protein was used to inhibit anti-CD59.
Seven cases of an isolated CD59 deficiency due to three distinct null alleles of the CD59 gene have been published so far. Recently we described the CD59-null allele c.146delA in a young child of heterozygous parents. Her plasma contained an alloantibody directed against the high-prevalence RBC antigen CD59. The antibody specificity was identified using soluble recombinant human CD59 protein, which blocked the reactivity of the patient's antibody and of monoclonal anti-CD59 but not of monoclonal anti-CD55. In addition, RBC alloantibodies such as anti-K, anti-C, anti-c, or anti-Fya remained unaffected. Therefore, inhibition by recombinant CD59 is a useful diagnostic tool to detect alloantibodies in the presence of anti-CD59.
This is the first demonstration of a human anti-CD59 alloantibody, which defines CD59 as an RBC blood group antigen. CD59 represents a candidate for a new blood group system.