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Effect of maternal iron status on the number of CD34+ stem cells harvested from umbilical cord blood

Authors

  • Belinda Pope,

    Corresponding author
    1. Pathology Department, Sydney Adventist Hospital, NSW, Australia
    2. Australasian Research Institute, Sydney Adventist Hospital, NSW, Australia
    3. Faculty of Medicine, University of New South Wales, NSW, Australia
    • Address correspondence to: Belinda Pope, Pathology Department, Sydney Adventist Hospital, 185 Fox Valley Road, Wahroonga, NSW 2076, Australia; e-mail: Belinda.pope@sah.org.au.

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  • Bevan Hokin,

    1. Pathology Department, Sydney Adventist Hospital, NSW, Australia
    2. Sydney Medical School, University of Sydney, NSW, Australia
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  • Ross Grant

    1. Australasian Research Institute, Sydney Adventist Hospital, NSW, Australia
    2. Faculty of Medicine, University of New South Wales, NSW, Australia
    3. Sydney Medical School, University of Sydney, NSW, Australia
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Abstract

Background

Hematopoietic progenitor cells (HPCs) from umbilical cord blood (UCB) are an alternative source of stem cells. However an adequate number of HPCs must be harvested from each UCB sample to make therapeutic applications possible. This study investigated the impact of selected maternal indices (in particular iron status) on the number of CD34+ cells collected in the UCB.

Study Design and Methods

Blood samples were collected from 91 matched mother and umbilical cord pairs and analyzed for full blood count, iron status, and C-reactive protein. Viable CD34 enumeration was performed on the cord blood samples.

Results

Low CD34+ cell counts were associated with higher maternal serum ferritin (SF), older mothers, lower UCB white blood cell count (WCC), lower UCB nucleated red blood cells (NRBCs), and lower birthweight. Maternal SF correlated with maternal variables of iron status and RBC indices, newborn weight, placental weight, and NRBCs/100 WCC.

Conclusion

This study indicates that lower numbers of CD34+ cells are more likely to occur when collected from mothers with higher SF. This finding suggests that maternal SF and associated iron status play a significant, but as yet undefined, role in the generation of CD34+ cells in UCB.

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