Aldehyde dehydrogenase–bright cells correlated with the colony-forming unit–granulocyte-macrophage assay of thawed cord blood units

Authors

  • Hye Ryun Lee,

    1. Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea
    Search for more papers by this author
  • Sue Shin,

    Corresponding author
    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
    3. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    • Address reprint requests to: Jong Hyun Yoon, MD, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea; e-mail: slice@paran.com; or Sue Shin, MD, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea; e-mail: Jeannie@snu.ac.kr

    Search for more papers by this author
    • SS and JHY contributed equally to this study as co-corresponding authors.
  • Jong Hyun Yoon,

    Corresponding author
    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
    3. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    • Address reprint requests to: Jong Hyun Yoon, MD, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea; e-mail: slice@paran.com; or Sue Shin, MD, Department of Laboratory Medicine, Seoul National University Boramae Hospital, Boramaro 5 gil 20, Dongjak-gu, 156-707, Seoul, Korea; e-mail: Jeannie@snu.ac.kr

    Search for more papers by this author
    • SS and JHY contributed equally to this study as co-corresponding authors.
  • Eun Youn Roh,

    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    2. Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea
    3. Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea
    Search for more papers by this author
  • Byoung Jae Kim,

    1. Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea
    Search for more papers by this author
  • Eun Young Song

    1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea
    Search for more papers by this author

  • This study was supported by a grant of the Research Project, Seoul Metropolitan Public Cord Blood Bank (2012-005).

Abstract

Background

The number of aldehyde dehydrogenase–bright (ALDHbr) cells has been suggested as a viable marker of hematopoietic stem cell function. We evaluated the suitability of ALDHbr cell analysis in the quality assessment of postthaw cord blood (CB) units.

Study Design and Methods

A total of 245 CB units were obtained for estimating the numbers of total nucleated cells (TNCs), CD34+ cells, ALDHbr cells, ALDHbr cells among CD34+ cells (CD34+ALDHbr cells), CD34+ cells among ALDHbr cells (ALDHbrCD34+ cells), colony-forming unit (CFU)–granulocyte-macrophages (GMs), and CFU–granulocyte-erythrocyte-macrophage-megakaryocytes (GEMMs). Simple linear regression analysis was performed to assess the correlation between the number of TNCs and CD34+ cells before and after crypreservation and CD34+ALDHbr cells, ALDHbr cells, and ALDHbr CD34+ cells after cryopreservation and the number of CFU-GEMMS and CFU-GMs.

Results

The number of CFU-GMs was found to be significantly correlated with the number of CD34+ cells before and after cryopreservation (r = 0.418 and r = 0.359, respectively), CD34+ALDHbr cells, ALDHbr cells, and ALDHbrCD34+ cells (r = 0.426, r = 0.455, and r = 0.469, respectively). The number of CFU-GEMMs was found to be significantly correlated with the number of TNCs and CD34+ cells before and after cryopreservation (TNCs, r = 0.251 and r = 0.250, respectively; CD34+ cells, r = 0.391 and r = 0.347, respectively), CD34+ALDHbr cells, ALDHbr cells, and ALDHbrCD34+ cells (r = 0.297, r = 0.297, and r = 0.252, respectively).

Conclusion

The high correlation found between ALDH activity and CFU-GM number supports the suitability of ALDH analysis in the quality assessment of postthaw CB units.

Ancillary