This work was funded in part by the Excellence Cluster REBIRTH (Unit 6.3).
Semaphorin 3A alters endothelial cell immunogenicity by regulating Class II transactivator activity circuits
Article first published online: 28 MAR 2014
© 2014 AABB
Volume 54, Issue 8, pages 1961–1970, August 2014
How to Cite
Schlahsa, L., Zhang, H., Battermann, A., Verboom, M., Immenschuh, S., Eiz-Vesper, B., Stripecke, R., Engelmann, K., Blasczyk, R. and Figueiredo, C. (2014), Semaphorin 3A alters endothelial cell immunogenicity by regulating Class II transactivator activity circuits. Transfusion, 54: 1961–1970. doi: 10.1111/trf.12631
- Issue published online: 15 AUG 2014
- Article first published online: 28 MAR 2014
- Manuscript Accepted: 16 DEC 2013
- Manuscript Revised: 12 DEC 2013
- Manuscript Received: 8 MAR 2013
- Excellence Cluster REBIRTH (Unit 6.3)
Fig. S1. CIITA overexpression enhances Sema3A receptor expression. HCECs were transfected with a CIITA-encoding vector and an empty control vector (pcDNA). (A) The effect of CIITA overexpression on the mRNA levels of HLA-DR and Sema3A receptors as determined by RT-PCR. Shown are the results of three independent experiments; significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, or ***p < 0.001). (B) Expression of Sema3A receptors was analyzed by flow cytometry. Displayed are the results of three independent experiments measuring the fold increase of mean fluorescence intensity in comparison to non-transfected cells. Statistical significance is indicated by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).
Table S1. Real Time (RT)-PCR primers and probes of candidate genes
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