Fig. S1. CIITA overexpression enhances Sema3A receptor expression. HCECs were transfected with a CIITA-encoding vector and an empty control vector (pcDNA). (A) The effect of CIITA overexpression on the mRNA levels of HLA-DR and Sema3A receptors as determined by RT-PCR. Shown are the results of three independent experiments; significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, or ***p < 0.001). (B) Expression of Sema3A receptors was analyzed by flow cytometry. Displayed are the results of three independent experiments measuring the fold increase of mean fluorescence intensity in comparison to non-transfected cells. Statistical significance is indicated by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).


Table S1. Real Time (RT)-PCR primers and probes of candidate genes

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