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trf12640-sup-0001-fig_s1.pdf224K

Fig. S1. Lipid mass spectrometry of platelet concentrates (PLCs) with added nHDL3 at concentrations to double the HDL3-cholesterol level in PLC-plasma. As control, PBS in similar volumes were added. Shown are significant delta differences (in %) in Day 5/Day 0 ratios of plasma lipid-species between added nHDL3 and control. Asterisks indicate significances * = p < 0.05, ** = p < 0.01, *** = p < 0.001. Cer = ceramide, Hex2Cer = di-hexosylceramide (most likely lactosylceramide), LPC = lysophosphatidyl-choline, PC = phosphatidylcholine, PE = phosphatidyl-ethanolamine, PC O,PC-plasmalogen, PE P = PE-plasmalogen.

trf12640-sup-0002-fig_s2.pdf209K

Fig. S2. Lipid mass spectrometry of platelet concentrates (PLCs) with added ApoA-I at concentrations to double the ApoA-I level in PLC-plasma. As control, PBS in similar volumes were added. Shown are Day 5/Day 0 ratios of A) platelet and B) plasma lipid classes. Asterisks indicate significances in Day 5/Day 0 ratio between added ApoA-I and control, with * = p < 0.05, ** = p < 0.01. FC = free cholesterol, CE = cholesteryl ester, SM = sphingomyelin, dhSM = dihydroSM, Cer = ceramide, LPC = lysophosphatidylcholine, PC = phosphatidylcholine, PS = phosphatidylserine, PE = phosphatidylethanolamine, PI = phosphatidylinositol, PC-O = PC-plasmalogen, PE P = PE-plasmalogen, HexCer = hexosylCer, Hex2Cer = di-hexosylCer (most likely lactosylceramide), SPH = sphingosine, SPH t18:0 = phytosphingosine, SPC = sphingosylphosphorylcholine, S1P = sphingosine-1-phosphate, LPA = lysophosphatidic acid, LPG = lysophosphatidylglycerol, PA = phosphatidic acid, PG = phosphatidylglycerol, BMP = bis(monoacylglycero)phosphate, CL = cardiolipin.

trf12640-sup-0003-fig_s3.pdf117K

Fig. S3. Lipid mass spectrometry of platelet concentrates (PLCs) with added nHDL3 at concentrations to double the HDL3-cholesterol level in PLC-plasma. As control, PBS in similar volumes were added. Shown are absolute levels (nmol/mg cell protein) of platelet lysophosphatidic acid (LPA)-species on Day 0 and Day 5. * = p < 0.05.

trf12640-sup-0004-fig_s4.pdf67K

Fig. S4. Nanoparticle Tracking Analysis (NTA) of platelet concentrates (PLCs) with added nHDL3 or ApoA-I to double the ApoA-I level in PLC plasma. Added PBS in similar volumes served as control. Shown are mean ± SD particle size (nm) on Day 0 and Day 5 from 6 individual experiments.

trf12640-sup-0005-table_s1.pdf65K

Table S1. Flow cytometric expression analysis of CD62P, CD41 and CD61 without stimulation (Basal) or upon TRAP-6 (10 μM) or ADP (5 μM) stimulation during 5 days platelet concentrate (PLC) storage. Mean fluorescence intensities (MFI) are shown. Changes are indicated as % of basal (100%). * = p < 0.05, ** = p < 0.01, compared to control. † = p < 0.05, compared to added apoA-I. Native (n)HDL3 or apoA-I were added in concentrations to double the apoA-I level in PLC plasma. Added PBS in a similar volume served as control.

trf12640-sup-0006-table_s2.pdf50K

Table S2. Lipid class ratios on Day 0 (D0) and Day 5 (D5) in A) platelets and B) plasma upon added apoA-I to double the apoA-I concentration in PLC-plasma. Added PBS in similar volumes served as control. *, **, *** = Significant change in ratio within the group, † (p < 0.05), †† (p < 0.01) = significant difference in ratio on Day 5 between control and upon added apoA-I. CE = cholesteryl ester, FC = free cholesterol, PC = phosphatidylcholine, LPC = lysophosphatidylcholine, LPA = lysophosphatidic acid, PA = phosphatidic acid, CL = cardiolipin, Cer = ceramide, SM = sphingomyelin, S1P = sphingosine-1-phosphate, PS = phosphatidylserine, PE P = PE-plasmalogen, PE = phosphatidylethanolamine.

trf12640-sup-0007-table_s3.pdf47K

Table S3. Lipid mass spectrometry data showing absolute amounts (nmol/mg cell protein) of platelet cholesteryl ester (CE)-species on Day 0 (D0) and Day 5 (D5) upon added native (n)HDL3 in a concentration that doubled the HDL3-cholesterol in platelet concentrate plasma. Control = PBS added in a similar volume as nHDL3. Shown are also changes in % from D0 to D5. * = p < 0.05, ** = p < 0.01 for D0-D5 change for respective sample, † = p < 0.05, †† = p < 0.01 between Control and nHDL3 on Day 0. b.d. = below detection limit (all replicates <0.1 nmol/mg).

trf12640-sup-0008-table_s4.pdf45K

Table S4. Lipid mass spectrometry data showing absolute amounts (nmol/mg cell protein) of platelet cholesteryl ester (CE)-species on Day 0 (D0) and Day 5 (D5) upon added apoA-I in a concentration that doubled the apoA-I concentration in platelet concentrate plasma. Control = PBS added in a similar volume as apoA-I. Shown are also changes in % from D0 to D5. * = p < 0.05, ** = p < 0.01 for D0-D5 change for respective sample, † = p < 0.05, †† = p < 0.01 between Control and nHDL3 on Day 0. b.d. = below detection limit (all replicates <0.1 nmol/mg).

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