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Appendix S1. Blood bank quality controls.

Fig. S1. Profiles of selected intra- and extracellular concentrations of metabolites involved in purine metabolism. Fold change was normalized to Day 0. Bars represent standard deviations.

Fig. S2. Profiles of selected intra- and extracellular concentrations of metabolites involved in TCA cycle. Fold change was normalized to Day 0. Bars represent standard deviations.

Fig. S3. Profiles of selected intra- and extracellular concentrations of metabolites involved in glutathione metabolism. Fold change was normalized to Day 0. Bars represent standard deviations.

Fig. S4. Profiles of selected intra- and extracellular concentrations of metabolites involved in glycolysis and pentose phosphate pathway. Fold change was normalized to Day 0. Bars represent standard deviations.

Fig. S5. Profiles of selected intra- and extracellular concentrations of metabolites involved in glycerophospholipid metabolism. Fold change was normalized to Day 0. Bars represent standard deviations.

Table S1. Summary of sample preparation for flow cytometry analysis

Table S2. In vitro measurements of apheresis platelet quality in PC samples stored for a period of 10 days. Unless otherwise noted, results are presented as mean ± standard deviation (n = 6)

Table S3. Markers of platelet activation and metabolism in apheresis platelets stored for a period of 10 days. Results are presented as mean ± standard deviation (n = 6)

Table S4. Targeted metabolites identified in the platelets metabolome

Table S5. Identified unexpected metabolites

Table S6. Metabolomics quality controls

Table S7. Pathway analysis of the endo-metabolome

Table S8. Pathway analysis of the exo-metabolome

Table S9. Variable contribution to PCA

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