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Monoclonal anti-A activity against the FORS1 (Forssman) antigen

Authors

  • Katie Barr,

    1. Biotech Innovation Centre, School of Engineering, Faculty of Design and Creative Technologies, Auckland University of Technology, Auckland, New Zealand
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  • Elena Korchagina,

    1. Shemyakin Institute of Bioorganic Chemistry, Moscow, Russian Federation
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  • Inna Popova,

    1. Shemyakin Institute of Bioorganic Chemistry, Moscow, Russian Federation
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  • Nicolai Bovin,

    1. Shemyakin Institute of Bioorganic Chemistry, Moscow, Russian Federation
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  • Stephen Henry

    Corresponding author
    1. Biotech Innovation Centre, School of Engineering, Faculty of Design and Creative Technologies, Auckland University of Technology, Auckland, New Zealand
    • Address reprint requests to: Stephen Henry, Biotech Innovation Centre, School of Engineering, Faculty of Design and Creative Technologies, AUT University, Private Bag 92006, Auckland 1142, New Zealand; e-mail: kiwi@aut.ac.nz.

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  • NB was supported by grant Molecular and Cell Biology of RAS Presidium.

Abstract

Background

The FORS blood group system (originally recognized as the Apae phenotype) was discovered by sporadic activity against polyclonal anti-A reagents and activity against the lectin Helix pomatia. The extent of monoclonal anti-A reagent activity against the FORS1 antigen is serologically and immunochemically incomplete.

Study Design and Methods

In the absence of natural FORS1-positive red blood cells (RBCs), kodecytes were created with synthetic disaccharide and pentasaccharide Forssman function-spacer-lipid (FSL) constructs, Fsdi-kodecytes, and FORS1-kodecytes, respectively. FSL constructs were also applied to solid surfaces and used in solid-phase enzyme immunoassays. A range of characterized monoclonal anti-A and anti-B reagents were then serologically and immunochemically characterized against these Forssman antigens. Polyclonal human anti-A, anti-B, the lectin H. pomatia serologic reagents; and canine RBCs were used as serologic controls.

Results

None of 19 different monoclonal anti-A reagents were able to detect the pentasaccharide Forssman on FORS1-kodecytes, while three reagents were able to detect disaccharide Forssman on Fsdi-kodecytes. Most anti-A reagents were immunochemically reactive with both the di- and the pentasaccharide Forssman antigens in the solid-phase assays. Historic polyclonal human anti-A and the lectin H. pomatia reacted strongly with the FORS1-kodecytes, correlating with the discovery of the Apae phenotype and supporting the use of FORS1-kodecytes as FORS1 surrogates.

Conclusions

Monoclonal anti-A reagents, despite showing reactivity against the FORS1 antigen in solid-phase assays are unlikely to cause the agglutination of FORS1 antigen–positive RBCs.

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