Get access

The genomic and seroprevalence of human bocavirus in healthy Chinese plasma donors and plasma derivatives

Authors

  • Hongxue Li,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Miao He,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Peibin Zeng,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Zhan Gao,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Guohui Bian,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Chunhui Yang,

    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    Search for more papers by this author
  • Wuping Li

    Corresponding author
    1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    2. Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
    • Address reprint requests to: Wuping Li, Hua Cai Road 26 Hao, Dong San Huan Road Er Duan, Chengdu, Sichuang 610052, China; e-mail: lwpzhr@sina.com.

    Search for more papers by this author

  • This study was supported by the basic grant of Institute of Pathogen Biology, Chinese Academy of Medical Sciences (Grant 2011IPB106).

Abstract

Background

Human bocavirus (HBoV) is a novel parvovirus identified in 2005. It has mostly been detected in respiratory and enteric infections and has not been studied large scale in blood products in relation to transfusion.

Study Design and Methods

An in-house quantitative polymerase chain reaction (Q-PCR) was developed to test HBoV DNA in plasma and plasma derivatives. Plasma samples (n = 6096) collected from healthy donors, 241 plasma pools, and 326 plasma derivatives were screened for HBoV DNA by Q-PCR. Positive samples were confirmed by nested PCR and further amplified for sequence analysis and phylogenetic studies. The prevalence of immunoglobulin (Ig)G and IgM specific to HBoV structural proteins was measured by enzyme-linked immunosorbent assay in 209 samples grouped according to virus load (Group 1, HBoV DNA >104 copies/mL; Group 2, HBoV DNA >5 × 102 copies/mL but below 104 copies/mL; Group 3,HBoV DNA negative).

Results

The genomic prevalence of HBoV in the plasma donors was 9.06%, ranging from 5.01 × 102 to 3.02 × 106 copies/mL. HBoV-specific IgG and IgM were detected at 20.00 and 7.50% in Group 1, at 20.29 and 2.90% in Group 2, and at 13.00 and 4.0% in Group 3, respectively. Phylogenetic analyses proved that HBoV Genotype 1 was the prevalent genotype in Chinese plasma donors.

Conclusion

Low levels of HBoV DNA were detectable at high prevalence in Chinese plasma donors and plasma derivatives. Further study is needed to determine whether HBoV screening is necessary.

Ancillary