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The value of molecular expression of KIT and KIT ligand analysed using real-time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours

Authors

  • T. A. Costa Casagrande,

    Corresponding author
    1. Biotechnology Master Program, Positivo University, Curitiba, Paraná, Brazil
    • Correspondence address:

      T. A. Costa Casagrande

      Department of Surgery Faculty of Veterinary Medicine and Animal Science

      University of São Paulo

      Av. Prof. Orlando Marques de Paiva

      87 CEP 05508-270 São Paulo, SP, Brazil

      e-mail: thaiscosta@up.com.br

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  • L. M. de Oliveira Barros,

    1. Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
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  • H. Fukumasu,

    1. Department of Basic Sciences, School of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil
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  • B. Cogliati,

    1. Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
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  • L. M. Chaible,

    1. Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
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  • M. L. Z. Dagli,

    1. Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
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  • J. M. Matera

    1. Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil
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Abstract

This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) and the rate of tumour recurrence and tumour-related deaths in dogs affected with mast cell tumour (MCT). Kaplan–Meier curves were constructed to compare tumour recurrence and tumour-related death between patients. The log-rank test was used to check for significant differences between curves. KIT-I, KIT-II and KIT-III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour-related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT-PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis.

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