Get access

Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours

Authors

  • S. Abou Asa,

    1. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt
    Search for more papers by this author
  • T. Mori,

    1. Laboratory of Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    2. Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan
    Search for more papers by this author
  • K. Maruo,

    1. Laboratory of Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    2. Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan
    Search for more papers by this author
  • A. Khater,

    1. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt
    Search for more papers by this author
  • A. El-sawak,

    1. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    Search for more papers by this author
  • E. Abd el-Aziz,

    1. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt
    Search for more papers by this author
  • T. Yanai,

    1. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    Search for more papers by this author
  • H. Sakai

    Corresponding author
    1. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan
    2. Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan
    • Correspondence address:

      H. Sakai

      Laboratory of Veterinary Pathology

      Department of Veterinary Medicine

      Faculty of Applied Biological Sciences

      Gifu University

      1-1 Yanagido

      Gifu 501-1193, Japan

      e-mail: shiroki@gifu-u.ac.jp

    Search for more papers by this author

Abstract

We examined whether mutation of the platelet-derived growth factor receptor protein tyrosine kinase (PDGFR)-α and PDGFR-β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans- or juxtamembrane regions of PDGFR-α and PDGFR-β were analysed with immunohistochemical staining and polymerase chain reaction-direct sequencing using DNA from paraffin-embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR-α and almost most of the HA cases were negative for PDGFR-β. Of the HSA cases, 55.6% were negative for PDGFR-α and 63% were strongly positive for PDGFR-β. Among the HA cases, 1 missense mutation was detected in PDGFR-α exon 18 and 1 in PDGFR-β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR-β. Thus, genomic mutation of trans- or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.

Get access to the full text of this article

Ancillary