Evaluation of hematologic, blood gas, and select biochemical variables in ovine whole blood stored in CPDA-1 bags
Version of Record online: 31 DEC 2012
© 2012 American Society for Veterinary Clinical Pathology
Veterinary Clinical Pathology
Volume 42, Issue 1, pages 27–30, March 2013
How to Cite
Sousa, R. S., Barrêto-Júnior, R. A., Sousa, I. K. F., Chaves, D. F., Soares, H. S., Barros, I. O., Minervino, A. H. H. and Ortolani, E. L. (2013), Evaluation of hematologic, blood gas, and select biochemical variables in ovine whole blood stored in CPDA-1 bags. Veterinary Clinical Pathology, 42: 27–30. doi: 10.1111/vcp.12014
- Issue online: 4 MAR 2013
- Version of Record online: 31 DEC 2012
- Blood transfusion;
- plasma hemoglobin;
- red blood cell metabolism
The economic consequences from mortality of sheep after blood loss can be considerable. To date there are no reports addressing hematologic, blood gas, and biochemical changes in ovine blood stored in CPDA-1 bags.
The aim of this study was to investigate hematologic, blood gas, and biochemical alterations resulting from storage of ovine blood in CPDA-1 bags to establish transfusion protocols in sheep.
From each of 7 healthy 8-month-old sheep 450 mL of blood were collected into CPDA-1 bags and stored for 35 days in at 3–6°C. Samples were taken from the bags at days 0, 7, 14, 21, and 35. Whole blood was used to assess PCV, MCV, RBC count, pH, pO2, pCO2, and concentrations of bicarbonate, sodium, and lactate. Plasma was used to measure potassium, hemoglobin, and glucose concentrations.
The PCV remained stable throughout the storage period, while plasma hemoglobin and MCV began to increase on days 7 and 21, respectively. The RBC count began to decrease on day 21. Blood pH decreased and pCO2 increased steadily throughout the storage period. Potassium concentration increased from 3.8 to 18.3 mmol/L on day 7 and remained high thereafter. In contrast, sodium concentration began to decrease on day 7.
The results show that ovine blood undergoes hematologic, blood gas, and biochemical changes during storage. Further studies are required to establish RBC viability in CPDA-1 bags after a storage period of 35 days.