Characterization of the aggregation responses of camel platelets
Article first published online: 6 AUG 2013
© 2013 American Society for Veterinary Clinical Pathology
Veterinary Clinical Pathology
Volume 42, Issue 3, pages 307–313, September 2013
How to Cite
Ghumlas, A. K. A. and Gader, A. G. M. A. (2013), Characterization of the aggregation responses of camel platelets. Veterinary Clinical Pathology, 42: 307–313. doi: 10.1111/vcp.12062
- Issue published online: 13 SEP 2013
- Article first published online: 6 AUG 2013
- Deanship of research
- King Saud University
- Riyadh and King Abdul Aziz City for Science and Technology. Grant Number: (KACST Project # AT-15-77)
- P2Y12 receptor;
- platelet activation
Despite evidence of active hemostasis, camel platelets barely respond to common aggregating agents at standard doses used for human platelet aggregation.
The purpose of the study was to find out whether camel platelets can be activated by high doses or combinations of aggregation agonists, and to characterize the receptor that mediates the aggregation response to adenosine diphosphate (ADP), the most potent agonist for camel platelets known so far.
Aggregation studies were performed with platelet-rich plasma (PRP) in response to multiple doses or combinations of ADP, epinephrine (EPN), collagen, and arachidonic acid (AA). Aggregation responses to ADP were performed before and after the addition of the ADP receptor (P2Y12) antagonist Clopidogrel.
Camel platelets responded to ADP at doses higher than the standard dose for human platelets, and to combinations of EPN and other agonists, while no aggregation was elicited with EPN or AA alone. Clopidogrel blocked the ADP-induced aggregation responses in a dose-dependent fashion in vitro.
Camel platelet aggregation can be activated by increasing the dose of some agonists such as ADP, but not AA or EPN. Irreversible aggregation of camel platelets could also be triggered by a combination of EPN and ADP, and collagen and AA. Inhibition with clopidogrel suggests that camel platelets express the ADP receptor, P2Y12. Understanding platelet function in camels will add to the understanding of platelet function in health and disease.