Markers of lipid peroxidation and α-tocopherol levels in the blood and neoplastic tissue of dogs with malignant mammary gland tumors
Version of Record online: 30 JUL 2013
© 2013 American Society for Veterinary Clinical Pathology
Veterinary Clinical Pathology
Volume 42, Issue 3, pages 323–328, September 2013
How to Cite
Karayannopoulou, M., Fytianou, A., Assaloumidis, N., Psalla, D., Constantinidis, T. C., Kaldrymidou, E. and Koutinas, A. F. (2013), Markers of lipid peroxidation and α-tocopherol levels in the blood and neoplastic tissue of dogs with malignant mammary gland tumors. Veterinary Clinical Pathology, 42: 323–328. doi: 10.1111/vcp.12064
- Issue online: 13 SEP 2013
- Version of Record online: 30 JUL 2013
- Inflammatory cancer;
- oxidative burst;
- thiobarbituric acid-reactive substances
Excess reactive oxygen species due to oxidative stress and the ensuing lipid peroxidation are believed to be involved in mammary gland tumor (MGT) pathogenesis.
The purpose of this study was to evaluate the extent of lipid peroxidation as evidenced by thiobarbituric acid-reactive substances (TBARS) formation, and the concentration of α-tocopherol as an inhibitor of lipid peroxidation, in blood and neoplastic tissue of dogs with malignant MGT. The correlation between inflammatory cell infiltration score and TBARS or α-tocopherol in MGT was also evaluated.
Sixteen intact female dogs with malignant MGT and 12 clinically healthy and age/weight-matched controls were included in the study. In all dogs, serum TBARS, α-tocopherol, total cholesterol, and triglycerides were measured. Tissue TBARS and α-tocopherol levels were determined in 1 cm3 sized tissue samples collected from MGT and adjacent, ipsilateral, normal mammary gland tissue from the 16 affected dogs. The degree of inflammatory cell tumor infiltration was evaluated histologically.
There were no statistically significant differences in serum levels of TBARS, α-tocopherol, total cholesterol, and triglycerides between dogs with and without malignant MGT. TBARS were significantly higher, whereas α-tocopherol was lower in neoplastic tissue when compared with normal mammary gland tissue. There was no correlation between TBARS or α-tocopherol concentration and the inflammatory cell infiltration score in neoplastic tissue.
The increased level of TBARS suggests oxidative stress induction in canine malignant MGT. The origin of this phenomenon is not clear, as a potential oxidative burst could not be attributed to inflammatory cells infiltrating the tumors.