Presented in part in abstract form at the 2011 American College of Veterinary Internal Medicine Forum, Denver, CO, June 2010.
Comparison of feline core bone marrow biopsies from different sites using 2 techniques and needles
Version of Record online: 28 JAN 2014
©2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology
Veterinary Clinical Pathology
Volume 43, Issue 1, pages 36–42, March 2014
How to Cite
Abrams-Ogg, A. C. G., Defarges, A. and Bienzle, D. (2014), Comparison of feline core bone marrow biopsies from different sites using 2 techniques and needles. Veterinary Clinical Pathology, 43: 36–42. doi: 10.1111/vcp.12108
Dr. Bienzle, an editor of the journal, was not involved in the peer-review process or the decision to publish this article.
- Issue online: 3 MAR 2014
- Version of Record online: 28 JAN 2014
- Ontario Veterinary College Pet Trust
- hematopoietic tissue;
Commonly used 11ga or 13ga biopsy needles are relatively large for cats and often preclude successful collection of bone marrow (BM) core biopsies.
The objective was to compare 15ga to 13ga BM core biopsy ease of collection and specimen quality.
In 10 cats, humeral biopsies obtained with 15ga EZ-IO needles were compared with iliac biopsies obtained with 13ga Jamshidi needles. Body condition, ease of collection, section quality, postprocedure pain, and swelling at biopsy sites were scored. Specimen length on mounted slides was measured and specimens with quality scores of 3–5 out of a maximum value of 5 were considered to be of acceptable diagnostic quality. The distribution of all parameters was assessed by Shapiro–Wilk tests, and differences in parameters were assessed by ANCOVA.
There were no significant differences between 15ga and 13ga biopsies, except that the 15ga humeral biopsy was judged to be easier to perform than 13ga iliac biopsy, and there was more severe postbiopsy swelling with 13ga biopsies. Facility score (mean ± SD), section quality score (median ± SD) and specimen length (mm, mean ± SD) were 12.7 ± 2.3, 2.0 ± 1.4, and 6.0 ± 2.1 for 15ga biopsies, respectively, and 8.9 ± 2.4, 1.0 ± 1.8, and 7.5 ± 2.5 for 13ga biopsies, respectively. Three specimens of acceptable quality were obtained with each 15ga and 13ga biopsies.
In cats, BM biopsy of the humerus with a 15ga needle is easier and causes less postbiopsy swelling than biopsy of the ilium with a 13ga needle. Sites and needles are equivalent with respect to yielding specimens of acceptable quality. Neither technique consistently captured high-quality specimens.