Effects of freezing on tissue factor activity in a thromboelastography assay
Article first published online: 10 JUL 2014
© 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology
Veterinary Clinical Pathology
Volume 43, Issue 3, pages 322–325, September 2014
How to Cite
Scruggs, J. L., Flatland, B. and Reed, A. (2014), Effects of freezing on tissue factor activity in a thromboelastography assay. Veterinary Clinical Pathology, 43: 322–325. doi: 10.1111/vcp.12171
- Issue published online: 3 SEP 2014
- Article first published online: 10 JUL 2014
- Clotting activator;
- quality control material;
- viscoelastic coagulation analysis
Human recombinant tissue factor (TF) can be used to activate viscoelastic coagulation assays. Although the package insert indicates that TF should not be frozen, published scientific data are not available. Ability to store frozen aliquots of TF would increase laboratory efficiency and decrease costs associated with performing TF-activated assays.
The objectives of this study were to determine the effects of freezing and storage time on TF's ability to activate commercially available quality control material in thromboelastography (TEG).
TF was diluted and frozen at −20°C and −70°C for 72 hours, one week, 2 weeks, 3 months, and 6 months, and used for TEG after thawing. TF activation of control material was also assessed after TF storage at room temperature for 0, 24, and 48 hours. Time to fibrin formation (R), rate of clot formation (K and angle α), and clot strength (MA) were measured, and ANOVA used to identify differences.
There were no significant differences in mean α and MA regardless of storage time or temperature. Means for R were not significantly increased at any time point after storage at room temperature or −70°C, but significant increases in mean R were observed after storage at −20°C starting after one week and continuing up to 6 months.
TF can be stored at room temperature for at least 48 hours, stored at −20°C for 72 hours, and stored at −70°C for up to 6 months without significant loss of activity for TEG.