Presented as an oral abstract at the International Veterinary Emergency and Critical Care Conference, Nashville, TN, September 2011.
Comparison of routine urinalysis and urine Gram stain for detection of bacteriuria in dogs
Article first published online: 14 JAN 2013
© Veterinary Emergency and Critical Care Society 2013
Journal of Veterinary Emergency and Critical Care
Volume 23, Issue 1, pages 23–28, January/February 2013
How to Cite
Way, L. I., Sullivan, L. A., Johnson, V. and Morley, P. S. (2013), Comparison of routine urinalysis and urine Gram stain for detection of bacteriuria in dogs. Journal of Veterinary Emergency and Critical Care, 23: 23–28. doi: 10.1111/vec.12012
The authors declare no conflicts of interest.
- Issue published online: 28 JAN 2013
- Article first published online: 14 JAN 2013
- Manuscript Accepted: 25 NOV 2012
- Manuscript Received: 6 FEB 2012
- bacterial cystitis;
To determine the utility of performing urine Gram stain for detection of bacteriuria compared to routine urine sediment examination and bacterial aerobic urine culture.
Prospective, observational study.
University teaching hospital.
Urine samples acquired via cystocentesis through convenience sampling from 103 dogs presenting to a tertiary referral institution.
All samples underwent routine urinalysis, including sediment examination, as well as urine Gram stain and quantitative bacterial aerobic urine culture.
Measurements and Main Results
The urine Gram stain demonstrated improved sensitivity (96% versus 76%), specificity (100% versus 77%), positive predictive value (100% versus 83%), and negative predictive value (93% versus 69%) when identifying bacteriuria, compared to routine urine sediment examination.
The urine Gram stain is highly sensitive and specific when detecting the presence of bacteria in canine urine samples. Gram staining should be considered when bacteriuria is highly suspected and requires rapid identification while bacterial culture is pending.