Ms. Wilson's current address: Highcroft Veterinary Referrals, Whitchurch, Bristol, United Kingdom.
In vitro study of the effect of dog food on the adsorptive capacity of activated charcoal
Article first published online: 20 MAR 2013
© Veterinary Emergency and Critical Care Society 2013
Journal of Veterinary Emergency and Critical Care
Volume 23, Issue 3, pages 263–267, May/June 2013
How to Cite
Wilson, H. E. and Humm, K. R. (2013), In vitro study of the effect of dog food on the adsorptive capacity of activated charcoal. Journal of Veterinary Emergency and Critical Care, 23: 263–267. doi: 10.1111/vec.12037
The authors declare no conflict of interest.
Funding provided by Department of Clinical Sciences and Services The Royal Veterinary College.
Presented in abstract form at the International Veterinary Emergency and Critical Care Symposium, Nashville, Tennessee, 2011.
- Issue published online: 4 JUN 2013
- Article first published online: 20 MAR 2013
- Manuscript Accepted: 6 FEB 2013
- Manuscript Received: 5 MAY 2012
- Department of Clinical Sciences and Services The Royal Veterinary College
To evaluate the effect of dog food on the adsorptive capacity of activated charcoal.
In vitro laboratory study.
University veterinary teaching hospital.
Materials and Methods
A fixed quantity of acetaminophen (50 mg) was added to a fixed quantity of activated charcoal (1 g), mixed with varying amounts of dog food (2–14 g). The admixture was agitated for 5 minutes, incubated at 38.5°C for 1 hour and then centrifuged for 30 minutes. The concentration of residual, nonadsorbed acetaminophen in the supernatant was quantitatively assayed by reverse phase high-pressure liquid chromatography with ultraviolet detection. Data were tested by linear regression analysis and statistical significance was set at P < 0.05.
Measurements and Main Results
A statistically significant reduction in the adsorptive capacity of activated charcoal was demonstrated with increasing amounts of dog food (R2 = 0.54; P = 0.0018). However, all measurements of residual acetaminophen were less than 100 mg/L, representing a reduction in acetaminophen concentration of more than 98.6%.
The addition of dog food to activated charcoal reduces its total adsorptive capacity for acetaminophen. However, this reduction in adsorptive capacity is unlikely to be clinically significant in the presence of both the formulation of dog food and the ratio of dog food to charcoal used in this study.