Brief Clinical Communication
Comparison of a gel column blood typing method and a point-of-care cartridge for Dog Erythrocyte Antigen 1.1
Financial support for this study provided by the Ontario Veterinary College Pet Trust Fund.
The authors declare no conflict of interests.
Address correspondence and reprint requests to
Dr. Shauna L. Blois, Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Blood typing for the presence of Dog Erythrocyte Antigen (DEA) 1.1 is recommended in all donor and recipient dogs prior to transfusion of blood products. The objective of this study was to determine if a point-of-care DEA 1.1 blood typing cartridge could be used in place of the gel column typing method.
Detection of DEA 1.1 was performed using a laboratory-based gel column method and a point-of-care cartridge. A convenience sample of 30 healthy blood donors, 13 dogs with immune-mediated hemolytic anemia (IMHA) (3 of which had concurrent immune-mediated thrombocytopenia [IMT]), and 44 dogs with other diseases was included in the study.
Agreement was observed between the tests for normal dogs and dogs with nonimmune-mediated disease in 74/74 cases. Two dogs in the IMHA group had indeterminate gel column blood typing results; 1 dog in this group had a negative gel column test result but a positive cartridge test result.
There was good agreement between the 2 methods for normal dogs and dogs with nonimmune-mediated disease. Blood typing methods in dogs with IMHA should be further investigated.