The authors declare no other conflict of interests.
Effect of syringe and aggregate filter administration on survival of transfused autologous fresh feline red blood cells
Article first published online: 13 NOV 2013
© Veterinary Emergency and Critical Care Society 2013
Journal of Veterinary Emergency and Critical Care
Volume 24, Issue 2, pages 162–167, March/April 2014
How to Cite
Heikes, B. W. and Ruaux, C. G. (2014), Effect of syringe and aggregate filter administration on survival of transfused autologous fresh feline red blood cells. Journal of Veterinary Emergency and Critical Care, 24: 162–167. doi: 10.1111/vec.12115
- Issue published online: 16 APR 2014
- Article first published online: 13 NOV 2013
- Manuscript Accepted: 30 SEP 2013
- Manuscript Received: 7 SEP 2012
- Morris Animal Foundation
- Department of Veterinary Clinical Sciences
- blood administration;
- flow cytometry;
To assess the effect of transfusion using a syringe and microaggregate filter on short-term survival and circulating half-life of autologous feline RBCs.
Prospective, internally controlled, observational study.
A University Teaching Hospital
Six apparently healthy, owned cats.
Blood collection by jugular venipuncture. Transfusion with labeled, autologous, fresh RBCs.
Measurements and Main Results
Anticoagulated whole blood (35 mL/cat) was collected in 2 equal aliquots. RBCs were washed and labeled at 2 different biotin densities, before suspension in autologous plasma. Labeled RBCs were then transfused using 2 methods, gravity flow and pump delivery using a 20 mL syringe and 18 μm microaggregate filter.
Whole blood samples were collected from each cat at 2-hour intervals for 12 hours following completion of the transfusions. Additional samples were collected at weekly intervals up to 6 weeks to assess circulating half-life of the transfused cells. Cell survival was assessed via flow cytometry. The proportion of transfused cells remaining in each of the 2 populations was measured.
Biotinylated RBCs were readily detected in all cats over the 6-week sampling period. There was a significant decrease in both populations of labeled cells over the 6-week period (P < 0.01), as expected. There was no difference in probability that the RBCs would survive up to 12 hours immediately following transfusion, and no significant difference in survival between the 2 groups over 6 weeks. The average half-life of all labeled cells was approximately 23 days.
We conclude that, in contrast to findings from dogs, transfusion of autologous feline RBCs using a syringe + aggregate filter method does not significantly impact short- or long-term survival of the transfused cells.