Cytokine concentration in stored canine erythrocyte concentrates

Authors

  • Raffaella Corsi DVM,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Maureen A. McMichael DVM, DACVECC,

    Corresponding author
    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
    • Address correspondence and reprint requests to Dr. Maureen A. McMichael, Department of Veterinary Clinical Sciences, University of Illinois at Urbana-Champaign, 1008 West Hazelwood Dr, Urbana, IL 61802. Email: mmcm@illinois.edu

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  • Stephanie A. Smith DVM, MS, DACVIM,

    1. Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Mauria O'Brien DVM, DACVECC,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Jennifer Herring DVM, DACVECC,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Thandeka R. Ngwenyama DVM,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Alyssa Galligan CVT,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Alison N. Beloshapka MS,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Ping Deng MS,

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • Kelly S. Swanson PhD

    1. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL
    2. Department of Animal Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Cham-paign, Urbana, IL
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  • The study was performed at the University of Illinois—Urbana campus.

  • The authors declare no conflicts of interests.

  • Presented in part at the European College of Veterinary Internal Medicine Congress, Seville, Spain, September 2011.

Abstract

Objective

To evaluate the effect of leukoreduction (LR) as compared to standard nonleukoreduced (NLR) units on cytokine concentrations in canine erythrocyte concentrates during regular storage time.

Design

Randomized, experimental study.

Setting

University teaching hospital.

Animals

Ten random-source research dogs.

Interventions

One unit of whole blood was collected from each dog and randomized to standard processing (NLR, n = 5) or prestorage LR (n = 5). All units were stored at 4°C. Samples were aseptically collected from each unit weekly for 5 weeks. On day 35, 1 mL of blood was collected from each unit and submitted for aerobic culture.

Measurement and Main Results

An ELISA assay was used to analyze the concentrations of IL-1β, IL-8, TNF-α, and IL-10. There were no significant effects of either group or storage time for IL-1β, IL-10, or TNF-α. IL-8 concentration was significantly increased over the storage period in NLR units, and was significantly higher compared to LR units on days 28 and 35. Aerobic culture was negative on all units.

Conclusions

This study demonstrated a marked, storage time-dependent accumulation of IL-8 in canine erythrocyte concentrates. Prestorage LR attenuated the accumulation of IL-8. This chemokine may contribute to the proinflammatory effects of transfusion of stored erythrocyte concentrates.

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