Anatomical location and culture of equine corneal epithelial stem cells
Article first published online: 28 MAY 2013
© 2013 American College of Veterinary Ophthalmologists
Volume 17, Issue 2, pages 106–112, March 2014
How to Cite
Moriyama, H., Kasashima, Y., Kuwano, A. and Wada, S. (2014), Anatomical location and culture of equine corneal epithelial stem cells. Veterinary Ophthalmology, 17: 106–112. doi: 10.1111/vop.12050
- Issue published online: 3 MAR 2014
- Article first published online: 28 MAY 2013
- corneal epithelial cells;
- corneal epithelium;
- localization of corneal epithelial stem cells;
- stem cell
To identify morphologically the locations of equine corneal epithelial stem cells (CESCs) and to culture these cells.
We studied the eyes of 12 adult thoroughbred horses.
Eye tissues were immunostained for two positive stem cell markers (p63, CK14) and one negative marker (CK3) to identify the locations of CESCs, so we could compare their immunostaining patterns with those of human stem cells previously reported. We compared the proliferation rates and morphological features of epithelial cells isolated from the corneal limbus and central cornea.
Undifferentiated cells expressing the same immunostaining pattern as human CESCs were present in the equine corneal limbus. Cultured epithelial cells isolated from the limbus expressed the same immunostaining pattern that CESCs show histologically, but cells isolated from the central cornea did not proliferate and could not be evaluated.
Equine CESCs were localized in the epithelial basal layer of the corneal limbus, where melanocytes reside. They could be cultured without loss of their undifferentiated nature. When collecting such stem cells, it may be useful to harvest and culture corneal epithelial tissues in the limbus where melanocytes serve as an indicator of the collecting area.