Altered fundus appearance resulting from autofluorescence imaging with the confocal scanning laser ophthalmoscope (cSLO) in cats

Authors

  • Laurence M. Occelli,

    1. Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, MI, USA
    Search for more papers by this author
  • Simon M. Petersen-Jones

    Corresponding author
    1. Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, MI, USA
    • Address communications to:

      S. M. Petersen-Jones

      Tel.: (517) 353-3278

      Fax: (517) 355-5164

      e-mail: peter315@cvm.msu.edu

    Search for more papers by this author

Abstract

Purpose

This paper is to report that imaging the tapetal fundus of cats with the 488 nm laser of the Spectralis® HRA+OCT (Heidelberg Engineering Inc., Heidelberg, Germany) can result in a pale appearance of the imaged area.

Animals studied and procedures

Wild-type and Rdy kittens (CRX mutant heterozygotes—CRXRdy+/−) (8–20 weeks of age) and adult cats (1–4 years of age) were imaged by confocal scanning laser ophthalmoscope (cSLO) and spectral domain optical coherence tomography (SD-OCT) using the Spectralis® HRA+OCT. Color fundus photography (RetCam II®, Clarity Medical Systems, Inc., Pleasanton, CA) was performed after imaging using the Spectralis® HRA+OCT.

Results

Following retinal cSLO imaging using the 488 nm laser (autofluorescence imaging) in both wild-type kittens and adult cats, the imaged region appeared paler than the adjacent retina that had not been imaged. This change was probably due to retinal bleaching and was fully reversible. Imaging CRXRdy+/− kittens or adults, which had very reduced levels of visual pigments, did not induce the altered fundus appearance.

Conclusions

Those using autofluorescence imaging by cSLO should be aware that it can induce a characteristic pale appearance of the tapetal fundus in the imaged area of normal cats.

Ancillary